Instituto de Óptica "Daza de Valdés," Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Universidad Politécnica de Madrid, Madrid, Spain.
Invest Ophthalmol Vis Sci. 2024 Jul 1;65(8):8. doi: 10.1167/iovs.65.8.8.
The purpose of this study was to evaluate the biomechanical and hydration differences in scleral tissue after two modalities of collagen cross-linking.
Scleral tissue from 40 adult white rabbit eyes was crosslinked by application of 0.1% Rose Bengal solution followed by 80 J/cm2 green light irradiation (RGX) or by application of 0.1% riboflavin solution followed by 5.4 J/cm2 ultraviolet A irradiation (UVX). Posterior scleral strips were excised from treated and untreated sclera for tensile and hydration-tensile tests. For tensile tests, the strips were subjected to uniaxial extension after excision. For hydration-tensile tests, the strips were dehydrated, rehydrated, and then tested. Young's modulus at 8% strain and swelling rate were estimated. ANOVAs were used to test treated-induced differences in scleral biomechanical and hydration properties.
Photo-crosslinked sclera tissue was stiffer (Young's modulus at 8% strain: 10.7 ± 4.5 MPa, on average across treatments) than untreated scleral tissue (7.1 ± 4.0 MPa). Scleral stiffness increased 132% after RGX and 90% after UVX compared to untreated sclera. Scleral swelling rate was reduced by 11% after RGX and by 13% after UVX. The stiffness of the treated sclera was also associated with the tissue hydration level. The lower the swelling, the higher the Young's modulus of RGX (-3.8% swelling/MPa) and UVX (-3.5% swelling/MPa) treated sclera.
Cross-linking with RGX and UVX impacted the stiffness and hydration of rabbit posterior sclera. The Rose Bengal with green light irradiation may be an alternative method to determine the efficacy and suitability of inducing scleral tissue stiffening in the treatment of myopia.
本研究旨在评估两种交联模式下巩膜组织的生物力学和水合差异。
用 0.1%玫瑰红溶液处理后，用 80 J/cm2 绿光辐照（RGX）或用 0.1%核黄素溶液处理后，用 5.4 J/cm2 紫外线 A 辐照（UVX）交联 40 只成年白兔眼的巩膜组织。从处理和未处理的巩膜中切取后巩膜条进行拉伸和水合拉伸测试。对于拉伸测试，条带在切除后进行单轴拉伸。对于水合拉伸测试，条带脱水、再水化，然后进行测试。估计应变 8%时的杨氏模量和溶胀率。采用方差分析检验处理后巩膜生物力学和水合特性的差异。
光交联巩膜组织比未处理的巩膜组织更硬（杨氏模量在 8%应变时：10.7±4.5 MPa，平均各处理）。与未处理的巩膜相比，RGX 后巩膜刚度增加了 132%，UVX 后增加了 90%。RGX 后巩膜的溶胀率降低了 11%，UVX 后降低了 13%。处理后的巩膜刚度也与组织水合水平有关。RGX（-3.8%肿胀/MPa）和 UVX（-3.5%肿胀/MPa）处理的巩膜肿胀越低，杨氏模量越高。
用 RGX 和 UVX 交联会影响兔后巩膜的刚度和水合作用。用孟加拉玫瑰红绿光照射可能是一种替代方法，用于确定巩膜组织强化治疗近视的疗效和适用性。
