Barron E T, Smyth P P, McDermott E W, Tobbia I N, Higgins N J
Department of Agriculture, Castleknock, Co. Dublin, Ireland.
Eur J Cancer. 1991;27(8):985-9. doi: 10.1016/0277-5379(91)90264-e.
Glucose-6-phosphate dehydrogenase (G6PD) activity was quantified cytochemically in mammary epithelial cells within frozen tissue sections from 38 patients with breast cancer and 44 with benign breast disease. G6PD activities were measured under atmospheres of both N2 and O2. The mean (S.E.) G6PD value 2.5 (0.23) IE U/min measured in N2 in mammary epithelial cells from the group of malignancies was significantly greater than that of 1.6 (0.37) IE U/min in the benign group (P less than 0.001), but there was considerable overlap between individual values. G6PD measured in O2 was detectable in 84% of malignancies compared to only 14% of benign biopsies and the group mean of 1.3 (0.18) IE U/min in the former was significantly greater than that of 0.35 (0.20) IE U/min in the latter (P less than 0.001). Significant correlations between G6PD activities measured in N2 and O2 were observed in both groups. The techniques present a sensitive method of identifying increases in G6PD activity in mammary epithelial cells and provide an assay that in a majority of cases permits the separation of malignant from benign tissues.
采用细胞化学方法对38例乳腺癌患者和44例乳腺良性疾病患者冷冻组织切片中的乳腺上皮细胞葡萄糖-6-磷酸脱氢酶（G6PD）活性进行了定量分析。分别在氮气和氧气环境下测定G6PD活性。恶性肿瘤组乳腺上皮细胞在氮气环境下测得的G6PD平均（标准误）值为2.5（0.23）国际酶单位/分钟，显著高于良性组的1.6（0.37）国际酶单位/分钟（P<0.001），但个体值之间存在相当大的重叠。在氧气环境下，84%的恶性肿瘤可检测到G6PD，而良性活检组织中仅14%可检测到，前者的组均值为1.3（0.18）国际酶单位/分钟，显著高于后者的0.35（0.20）国际酶单位/分钟（P<0.001）。两组在氮气和氧气环境下测得的G6PD活性之间均存在显著相关性。这些技术提供了一种灵敏的方法来识别乳腺上皮细胞中G6PD活性的增加，并提供了一种检测方法，在大多数情况下能够区分恶性组织和良性组织。
