Russell Hugh H, Sriskandan Shiranee
Department of Infectious Diseases, Faculty of Medicine, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK.
Microb Pathog. 2008 Jun;44(6):537-43. doi: 10.1016/j.micpath.2007.06.010. Epub 2008 Feb 6.
The superantigens, toxic shock syndrome toxin-1 (TSST-1) and staphylococcal enterotoxin B (SEB), were recently reported to repress global exoprotein synthesis in Staphylococcus aureus. To investigate if this phenomenon could be observed in a different Gram-positive pathogen, the effects of two major Streptococcus pyogenes superantigens on streptococcal secretome expression were examined. Using mutagenesis and genetic complementation, we demonstrated that neither streptococcal pyrogenic exotoxin A (SPEA) nor streptococcal mitogenic exotoxin Z (SMEZ) had any consistent effect on global protein expression or on transcription of genes encoding the secreted exoproteins, DNase B, SPEB and SPEG. In S. pyogenes, superantigen production does not appear to have a major regulatory role.
超抗原、中毒性休克综合征毒素-1（TSST-1）和葡萄球菌肠毒素B（SEB），最近有报道称它们会抑制金黄色葡萄球菌的整体外蛋白合成。为了研究在另一种革兰氏阳性病原体中是否能观察到这种现象，我们检测了两种主要的化脓性链球菌超抗原对链球菌分泌组表达的影响。通过诱变和基因互补，我们证明化脓性链球菌致热外毒素A（SPEA）和促有丝分裂链球菌外毒素Z（SMEZ）对整体蛋白表达或对编码分泌性外蛋白、DNase B、SPEB和SPEG的基因转录均无任何一致的影响。在化脓性链球菌中，超抗原的产生似乎没有主要的调节作用。
