Lill H, Nelson N
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Plant Mol Biol. 1991 Oct;17(4):641-52. doi: 10.1007/BF00037050.
The two operons atp1 and atp2, encoding the subunits of the F0F1 ATP-synthase, have been cloned and sequenced from the cyanobacterium Synechocystis sp. PCC 6803. The organization of the different genes in the operons have been found to resemble that of the cyanobacteria Synechococcus sp. PCC 6301 and Anabaena sp. PCC 7120. The Synechocystis F0F1 ATP-synthase has nine subunits. A tenth open reading frame with unknown function was detected at the 5' end of atp1, coding for a putative gene product similar to uncI in Escherichia coli. A promoter structure was inferred for the Synechocystis atp operons and compared to other known promoters of cyanobacteria. Even though the operon structure of atp1 and atp2 in Synechocystis resembles the corresponding operons of Synechococcus, the amino acid sequences of individual gene products show marked differences. Genetic distances between cyanobacterial genes and genes for ATP-synthase subunits from other species have been calculated and compiled into evolutionary trees.
已从集胞藻属PCC 6803蓝细菌中克隆并测序了编码F0F1 ATP合酶亚基的两个操纵子atp1和atp2。已发现操纵子中不同基因的组织类似于聚球藻属PCC 6301和鱼腥藻属PCC 7120蓝细菌的组织。集胞藻F0F1 ATP合酶有九个亚基。在atp1的5'端检测到一个功能未知的第十个开放阅读框，其编码的推定基因产物类似于大肠杆菌中的uncI。推断出集胞藻atp操纵子的启动子结构，并与蓝细菌的其他已知启动子进行了比较。尽管集胞藻中atp1和atp2的操纵子结构类似于聚球藻属的相应操纵子，但单个基因产物的氨基酸序列存在明显差异。已计算蓝细菌基因与其他物种ATP合酶亚基基因之间的遗传距离，并将其汇编成进化树。
