Zhang Wei, Wei Bofeng, Li Shoujiang, Wang Yueming, Wang Shaoyan
School of Chemical Engineering, University of Science and Technology, Anshan 114051, Liaoning, China.
Liaoning Provincial Key Laboratory of Fine Separation Technique, University of Science and Technology, Anshan 114051, Liaoning, China.
Polymers (Basel). 2017 Jun 9;9(6):214. doi: 10.3390/polym9060214.
The application of molecular imprinting technology in the separation and purification of active ingredients in natural products was widely reported, but remains a challenge. Enrichment and separation are especially limited. A surface imprinting technique was reported to synthesize molecularly imprinted microspheres (MIMs) in this article. With paeoniflorin (PF) as the template molecule, β-cyclodextrin (β-CD) and acrylamide (AA) as the functional monomers, and poly(glycidyl methacrylate, GMA) microspheres (P) as the backing material. MIMs have been characterized by FTIR and FESEM. Adsorption experiments indicated the adsorption capacity of MIMs was superior to those comparative non-imprinted microspheres (NIMs) and the binding isotherm of MIMs was in good agreement with the two-site binding model. The baseline separation of PF and its structural analogue albiflorin (AF) were achieved on the new MIMs packed column. MIMs showed good affinity and efficiency for separation of PF and AF compared with those comparative NIMs. The approach of fabricating MIMs is simple, rapid, and inexpensive, and may shed new light on the application of MIMs as a liquid chromatography stationary phase to separate and analyze PF and AF from the Red peony root extracts.
分子印迹技术在天然产物活性成分分离纯化中的应用已有广泛报道，但仍具挑战性。富集和分离尤其受限。本文报道了一种表面印迹技术，用于合成分子印迹微球（MIMs）。以芍药苷（PF）为模板分子，β-环糊精（β-CD）和丙烯酰胺（AA）为功能单体，聚甲基丙烯酸缩水甘油酯（GMA）微球（P）为载体材料。通过傅里叶变换红外光谱（FTIR）和场发射扫描电子显微镜（FESEM）对MIMs进行了表征。吸附实验表明，MIMs的吸附能力优于对比的非印迹微球（NIMs），且MIMs的结合等温线与双位点结合模型吻合良好。在装填新MIMs的色谱柱上实现了PF及其结构类似物 albiflorin（AF）的基线分离。与对比的NIMs相比，MIMs对PF和AF的分离显示出良好的亲和力和效率。制备MIMs的方法简单、快速且成本低廉，可能为MIMs作为液相色谱固定相从赤芍提取物中分离和分析PF和AF的应用提供新的思路。
