Skromne Isaac, Prince Victoria E
Department of Biology, University of Miami, Coral Gables, Florida 33146, USA.
Dev Dyn. 2008 Apr;237(4):861-82. doi: 10.1002/dvdy.21484.
Use of the zebrafish as a model of vertebrate development and disease has expanded dramatically over the past decade. While many articles have discussed the strengths of zebrafish forward genetics (the phenotype-driven approach), there has been less emphasis on equally important and frequently used reverse genetics (the candidate gene-driven approach). Here we review both current and prospective reverse genetic techniques that are applicable to the zebrafish model. We include discussion of pharmacological approaches, popular gain-of-function and knockdown approaches, and gene targeting strategies. We consider the need for temporal and spatial control over gain/loss of gene function, and discuss available and developing techniques to achieve this end. Our goal is both to reveal the current technical advantages of the zebrafish and to highlight those areas where work is still required to allow this system to be exploited to full advantage.
在过去十年中，斑马鱼作为脊椎动物发育和疾病模型的应用急剧增加。虽然许多文章讨论了斑马鱼正向遗传学（表型驱动方法）的优势，但对同样重要且常用的反向遗传学（候选基因驱动方法）的强调较少。在这里，我们综述了适用于斑马鱼模型的当前和未来反向遗传技术。我们讨论了药理学方法、常用的功能获得和敲低方法以及基因靶向策略。我们考虑了对基因功能的获得/丧失进行时空控制的必要性，并讨论了实现这一目标的现有和正在发展的技术。我们的目标既是揭示斑马鱼当前的技术优势，也是突出那些仍需开展工作以使该系统得到充分利用的领域。
