聚（dG）-聚（dC）-金纳米颗粒缀合物的合成与原子力显微镜表征

Synthesis and AFM characterization of poly(dG)-poly(dC)-gold nanoparticle conjugates.

作者信息

Zikich Dragoslav, Borovok Natalia, Molotsky Tatiana, Kotlyar Alexander

机构信息

Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, The Center for Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, 69978.

出版信息

Bioconjug Chem. 2010 Mar 17;21(3):544-7. doi: 10.1021/bc900527a. Epub 2010 Feb 18.

DOI:10.1021/bc900527a

PMID:20166677

Abstract

In the present work, we have synthesized conjugates between the 5 nm gold nanoparticles (Au-NP) and 5' thiol-functionalized, 700 bp poly(dG)-poly(dC). We have completely separated and purified to homogeneity conjugates bearing different number of poly(dG)-poly(dC) molecules per Au-NP by electrophoresis and HPLC. The conjugates were directly visualized by atomic force microscopy. We have demonstrated that Au NP-bound poly(dG)-poly(dC) can be considerably extended by Klenow exo(-) polymerase in the presence of dCTP and dGTP.

摘要

在本研究中，我们合成了5纳米金纳米颗粒（Au-NP）与5'硫醇功能化的700碱基对聚（dG）-聚（dC）之间的缀合物。我们通过电泳和高效液相色谱法将每个金纳米颗粒携带不同数量聚（dG）-聚（dC）分子的缀合物完全分离并纯化至同质状态。通过原子力显微镜直接观察到了这些缀合物。我们已经证明，在存在dCTP和dGTP的情况下，与金纳米颗粒结合的聚（dG）-聚（dC）可以被Klenow外切酶（-）聚合酶显著延长。