Jin Gui, Cheng Qiaoyuan, Feng Jian, Li Fanzhu
Department of Pharmaceutics, Zhejiang Chinese Medical University, Hangzhou 310053, PR China.
J Chromatogr Sci. 2008 Mar;46(3):276-87. doi: 10.1093/chromsci/46.3.276.
In vivo sampling of interstitial fluid using microdialysis (MD) fibers has become a standard and accepted procedure. The resulting small volume samples, often with low concentrations of the analyte of interest, present a particular challenge to analytical methods. Rapid developments of analytical techniques with high sensitivity accelerate their combination with MD. On-line MD-based analytical systems are receiving increasing attention because they can provide near real-time data prior to the off-line system. The purpose of this review is to provide information for on-line MD-analytical systems. Special emphasis has been given to the main progression on methodologies of each method during recent years. The advantages, limitations, and adaptivity are also discussed. These methods include on-line MD-liquid chromatography, on-line MD-capillary electrophoresis, on-line MD-mass spectrometry, and on-line MD-biosensor.
使用微透析（MD）纤维对间质液进行体内采样已成为一种标准且被认可的程序。由此产生的小体积样本，通常目标分析物浓度较低，这对分析方法构成了特殊挑战。高灵敏度分析技术的快速发展加速了它们与微透析的结合。基于在线微透析的分析系统正受到越来越多的关注，因为它们能够在离线系统之前提供近实时数据。本综述的目的是为在线微透析分析系统提供信息。特别强调了近年来每种方法在方法学上的主要进展。还讨论了其优点、局限性和适应性。这些方法包括在线微透析-液相色谱法、在线微透析-毛细管电泳法、在线微透析-质谱分析法和在线微透析-生物传感器法。
