让心灵的微光闪耀：神经化学检测的进展与未来方向。
Letting the little light of mind shine: Advances and future directions in neurochemical detection.
机构信息
Biomedical Engineering Graduate Group, University of California, Davis, Davis, CA, 95616, USA.
Neuroscience Graduate Group, University of California, Davis, Davis, CA, 95618, USA.
出版信息
Neurosci Res. 2022 Jun;179:65-78. doi: 10.1016/j.neures.2021.11.012. Epub 2021 Nov 30.
Synaptic transmission via neurochemical release is the fundamental process that integrates and relays encoded information in the brain to regulate physiological function, cognition, and emotion. To unravel the biochemical, biophysical, and computational mechanisms of signal processing, one needs to precisely measure the neurochemical release dynamics with molecular and cell-type specificity and high resolution. Here we reviewed the development of analytical, electrochemical, and fluorescence imaging approaches to detect neurotransmitter and neuromodulator release. We discussed the advantages and practicality in implementation of each technology for ease-of-use, flexibility for multimodal studies, and challenges for future optimization. We hope this review will provide a versatile guide for tool engineering and applications for recording neurochemical release.
通过神经化学释放进行的突触传递是整合和传递大脑中编码信息以调节生理功能、认知和情绪的基本过程。为了揭示信号处理的生化、生物物理和计算机制，需要精确测量具有分子和细胞类型特异性和高分辨率的神经化学释放动力学。在这里，我们综述了分析、电化学和荧光成像方法的发展，以检测神经递质和神经调质的释放。我们讨论了每种技术的优点和实用性，包括易用性、多模态研究的灵活性以及未来优化的挑战。我们希望这篇综述将为记录神经化学释放的工具工程和应用提供一个通用的指南。
Zotero 插件