Freysdottir J, Sigfusson A
Department of Immunology, National University Hospital, Landspitalinn, Reykjavik, Iceland.
J Immunol Methods. 1991 Aug 28;142(1):45-52. doi: 10.1016/0022-1759(91)90291-m.
A flow cytometric assay (FCA) was developed to measure complement receptor 1 (CR1) and the complement fragments C3d and C4d on erythrocytes. It was possible to measure these parameters accurately with intra- and interassay coefficients of variation of 2.0% and 6.5% respectively. The method was able to discriminate between low and high levels of erythrocyte CR1, C3d and C4d. Comparison with a previously described RIA method gave excellent correlation coefficients with r2 values of 0.94, 0.93 and 0.91 for CR1, C3d and C4d respectively. The flow cytometric assay was used to measure CR1, C3d and C4d on the erythrocytes of 98 healthy individuals and the 95% upper limits for C3d and C4d were established. There was a wide distribution of CR1 levels amongst these individuals but their C3d and C4d levels were low and often not above background. The possible application of this method in clinical medicine is discussed.
开发了一种流式细胞术检测法（FCA）来测量红细胞上的补体受体1（CR1）以及补体片段C3d和C4d。能够分别以2.0%和6.5%的批内和批间变异系数准确测量这些参数。该方法能够区分红细胞CR1、C3d和C4d的低水平和高水平。与先前描述的放射免疫分析法（RIA）方法进行比较，CR1、C3d和C4d的相关系数分别为r2值0.94、0.93和0.91，相关性良好。流式细胞术检测法用于测量98名健康个体红细胞上的CR1、C3d和C4d，并确定了C3d和C4d的95%上限。这些个体中CR1水平分布广泛，但他们的C3d和C4d水平较低，且通常不高于背景值。讨论了该方法在临床医学中的可能应用。
