Bachiega João C, Blanco Miriam M, Perez-Mendes Patrícia, Cinini Simone Maria, Covolan Luciene, Mello Luiz E
Department of Physiology, Universidade Federal de São Paulo, Sao Paulo, Brazil.
Epilepsy Behav. 2008 Jul;13(1):70-6. doi: 10.1016/j.yebeh.2008.02.010. Epub 2008 Mar 11.
This study was designed to characterize seizures induced with pentylenetetrazol (PTZ) in marmosets. Thirteen adult marmosets (Callithrix sp.) received 20, 30, or 40 mg/kg of PTZ intraperitoneally. PTZ caused all animals to switch their natural behavioral repertoire to early convulsive behavior. Seizure scores were low at lower PTZ doses, whereas the highest dose of PTZ led to seizure scores IV and V (according to Racine's scale) in 69% of animals. To further characterize the model we performed a preliminary evaluation of the efficacy of three antiepileptic drugs: phenobarbital, phenytoin, and carbamazepine. Phenobarbital prevented PTZ-induced seizures in 100% of trials. As expected, phenytoin and carbamazepine were not effective against PTZ-induced seizures. The present study describes the PTZ model of seizures in marmosets with a drug-response profile similar to that of the rodent model, thus bringing to a well-known model (PTZ in rodents) the complexity of a nonhuman primate brain.
本研究旨在描述戊四氮（PTZ）诱发狨猴癫痫发作的特征。13只成年狨猴（绢毛猴属）腹腔注射20、30或40mg/kg的PTZ。PTZ使所有动物的自然行为模式转变为早期惊厥行为。较低剂量的PTZ导致的癫痫发作评分较低，而最高剂量的PTZ使69%的动物出现IV级和V级癫痫发作评分（根据拉辛量表）。为了进一步描述该模型，我们对三种抗癫痫药物：苯巴比妥、苯妥英和卡马西平的疗效进行了初步评估。苯巴比妥在100%的试验中预防了PTZ诱发的癫痫发作。正如预期的那样，苯妥英和卡马西平对PTZ诱发的癫痫发作无效。本研究描述了狨猴的PTZ癫痫模型，其药物反应特征与啮齿动物模型相似，从而将非人类灵长类动物大脑的复杂性引入了一个著名的模型（啮齿动物的PTZ模型）。
