Bergmann-Leitner Elke S, Duncan Elizabeth H, Burge John Robert, Spring Michele, Angov Evelina
US Military Malaria Vaccine Program, Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Am J Trop Med Hyg. 2008 Mar;78(3):468-71.
To date, no immune correlates for blood stage-specific immunity against Plasmodium falciparum malaria parasites have been identified. Growth and/or invasion inhibition assays using sera from Phase 2a/b trials will aid in determining whether correlations with protective immunity can be established for these assays. A major constraint in the ability to evaluate functional antibody activities from populations in endemic areas is the relatively limited availability of sufficient sample quantity. For this reason, we developed a miniaturized and high-throughput method to measure growth inhibitory activity by quantification of parasite lactate dehydrogenase (pLDH) in a 384-microtiter plate format. This culture method can be extended beyond the pLDH-based readout to other techniques commonly used to determine growth/invasion inhibition.
迄今为止，尚未发现针对恶性疟原虫疟疾寄生虫血期特异性免疫的免疫相关指标。使用2a/2b期试验血清进行的生长和/或入侵抑制试验，将有助于确定这些试验能否建立与保护性免疫的相关性。评估流行地区人群功能性抗体活性能力的一个主要限制是，足够样本量的可用性相对有限。因此，我们开发了一种小型化高通量方法，通过在384孔微量滴定板中定量寄生虫乳酸脱氢酶（pLDH）来测量生长抑制活性。这种培养方法可以扩展到基于pLDH的读数之外，应用于其他常用于确定生长/入侵抑制的技术。
