Urdaneta-Marquez Ludmel, Bosio Christopher, Herrera Flor, Rubio-Palis Yasmin, Salasek Michael, Black William C
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1682, USA.
Am J Trop Med Hyg. 2008 Mar;78(3):479-91.
A population genetic analysis of gene flow was conducted among 619 Aedes aegypti from nine collections distributed among six geographic regions of Venezuela. Genetic markers included a 387-basepair region of the mitochondrial NADH dehydrogenase 4 (ND4) gene and single nucleotide polymorphisms (SNPs) at 11 nuclear loci. Genotypes at SNP loci were identified using melting curve analysis. Six different ND4 haplotypes were detected and patterns of variation suggested that collections were isolated by distance. The variance in SNP allele frequencies was much less than the variance in haplotype frequencies and a pattern of isolation by distance was not detected. Aedes aegypti from eight collections were orally challenged with dengue 2 virus. Disseminated infection rates ranged from 77% to 95%. The percentage of mosquitoes exhibiting a midgut infection barrier ranged from 2% to 15%, and those exhibiting a midgut escape barrier ranged from 2% to 18%. Venezuelan Ae. aegypti appear to be susceptible to dengue virus infection.
对来自委内瑞拉六个地理区域九个采集点的619只埃及伊蚊进行了基因流的群体遗传分析。遗传标记包括线粒体烟酰胺腺嘌呤二核苷酸脱氢酶4（ND4）基因的一个387个碱基对的区域以及11个核基因座的单核苷酸多态性（SNP）。使用熔解曲线分析鉴定SNP基因座的基因型。检测到六种不同的ND4单倍型，变异模式表明采集点按距离隔离。SNP等位基因频率的方差远小于单倍型频率的方差，未检测到按距离隔离的模式。对来自八个采集点的埃及伊蚊进行登革2型病毒口服攻击。传播感染率在77%至95%之间。表现出中肠感染屏障的蚊子百分比在2%至15%之间，表现出中肠逃逸屏障的蚊子百分比在2%至18%之间。委内瑞拉埃及伊蚊似乎对登革病毒感染敏感。
