在Liaison平台上进行的传染病血清学检测中防止检测干扰
Prevention of assay interference in infectious-disease serology tests done on the liaison platform.
作者信息
Berth Mario, Bosmans Eugene
机构信息
Algemeen Medisch Laboratorium, Immunology Department, Desguinlei 88, 2018 Antwerpen, Belgium.
出版信息
Clin Vaccine Immunol. 2008 May;15(5):891-2. doi: 10.1128/CVI.00012-08. Epub 2008 Mar 12.
Abstract
Immunoassay interference causing unexpected reactive results in magnetic-microparticle-based assays was detected. A systematic evaluation of Liaison Epstein-Barr virus immunoglobulin M showed that 5% of the positive results (0.4% of tested samples) could be explained by such interference. Adding chemical blocking reagents (polyvinylpyrrolidone and polyvinyl alcohol) to the assay buffers partially prevented this phenomenon.
摘要
检测到免疫测定干扰在基于磁性微粒的测定中导致意外的反应性结果。对Liaison Epstein-Barr病毒免疫球蛋白M的系统评估表明，5%的阳性结果（0.4%的测试样本）可归因于这种干扰。在测定缓冲液中添加化学封闭试剂（聚乙烯吡咯烷酮和聚乙烯醇）可部分防止这种现象。
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