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在Liaison平台上进行的传染病血清学检测中防止检测干扰

Prevention of assay interference in infectious-disease serology tests done on the liaison platform.

作者信息

Berth Mario, Bosmans Eugene

机构信息

Algemeen Medisch Laboratorium, Immunology Department, Desguinlei 88, 2018 Antwerpen, Belgium.

出版信息

Clin Vaccine Immunol. 2008 May;15(5):891-2. doi: 10.1128/CVI.00012-08. Epub 2008 Mar 12.

DOI:10.1128/CVI.00012-08
PMID:18337378
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2394835/
Abstract

Immunoassay interference causing unexpected reactive results in magnetic-microparticle-based assays was detected. A systematic evaluation of Liaison Epstein-Barr virus immunoglobulin M showed that 5% of the positive results (0.4% of tested samples) could be explained by such interference. Adding chemical blocking reagents (polyvinylpyrrolidone and polyvinyl alcohol) to the assay buffers partially prevented this phenomenon.

摘要

检测到免疫测定干扰在基于磁性微粒的测定中导致意外的反应性结果。对Liaison Epstein-Barr病毒免疫球蛋白M的系统评估表明，5%的阳性结果（0.4%的测试样本）可归因于这种干扰。在测定缓冲液中添加化学封闭试剂（聚乙烯吡咯烷酮和聚乙烯醇）可部分防止这种现象。

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Clin Chim Acta. 2008 Jan;387(1-2):150-2. doi: 10.1016/j.cca.2007.08.019. Epub 2007 Sep 8.
2
Rheumatoid factor interference in the determination of carbohydrate antigen 19-9 (CA 19-9).类风湿因子对糖类抗原19-9（CA 19-9）测定的干扰。
Clin Chem Lab Med. 2006;44(9):1137-9. doi: 10.1515/CCLM.2006.205.
3
Analysis of human sera that are polyreactive in an addressable laser bead immunoassay.在可寻址激光珠免疫分析中具有多反应性的人血清分析。
Clin Immunol. 2006 Sep;120(3):349-56. doi: 10.1016/j.clim.2006.03.007. Epub 2006 Apr 27.
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Suppression of non-specific binding in serological Luminex assays.血清学Luminex检测中非特异性结合的抑制
J Immunol Methods. 2006 Feb 20;309(1-2):200-4. doi: 10.1016/j.jim.2005.11.008. Epub 2005 Dec 19.
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J Clin Microbiol. 2005 Apr;43(4):1570-4. doi: 10.1128/JCM.43.4.1570-1574.2005.
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7
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Clin Chem. 2005 Jan;51(1):25-6. doi: 10.1373/clinchem.2004.042523. Epub 2004 Nov 11.
8
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J Clin Microbiol. 2002 May;40(5):1755-60. doi: 10.1128/JCM.40.5.1755-1760.2002.
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Resistance to nonspecific protein adsorption by poly(vinyl alcohol) thin films adsorbed to a poly(styrene) support matrix studied using surface plasmon resonance.利用表面等离振子共振研究吸附在聚（苯乙烯）支撑基质上的聚乙烯醇薄膜对非特异性蛋白质吸附的抗性。
Anal Chem. 2001 Nov 1;73(21):5232-9. doi: 10.1021/ac010368u.
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Interferences with two-site immunoassays by human anti-mouse antibodies formed by patients treated with monoclonal antibodies: comparison of different blocking reagents.单克隆抗体治疗患者产生的人抗小鼠抗体对双位点免疫分析的干扰：不同封闭试剂的比较
Clin Chem. 1998 Aug;44(8 Pt 1):1742-4.