Blum Miriam, Dolnikowski Gregory, Seyoum Elias, Harris Susan S, Booth Sarah L, Peterson James, Saltzman Edward, Dawson-Hughes Bess
Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St, Boston, MA, 02111, USA.
Endocrine. 2008 Feb;33(1):90-4. doi: 10.1007/s12020-008-9051-4. Epub 2008 Mar 13.
The literature describing vitamin D content of fat tissue is extremely limited. We conducted a pilot study that measured the concentrations of vitamin D(3) in the fat tissue and serum of obese adults. These measurements were performed using a new liquid chromatography mass spectrometry (LC/MS) method. The objectives of this study were: to measure and report the vitamin D(3) concentration in serum and subcutaneous fat samples from obese individuals and to examine the association of vitamin D(3) in fat with vitamin D(3) in serum. This cross-sectional study was conducted in 17 obese men and women who were scheduled to undergo gastric bypass surgery. The mean vitamin D(3) concentration in subjects' subcutaneous fat tissue samples was 102.8 +/- 42.0 nmol/kg. The mean vitamin D(3) concentration in serum was 7.78 +/- 3.99 nmol/l. Vitamin D(3) concentrations of fat tissue and serum were positively correlated (r = 0.68, P = 0.003). Consistent with previous findings in obese subjects, subjects in this study had suboptimal vitamin D status as demonstrated by a mean 25-hydroxyvitamin D concentration of 43.3 +/- 15.4 nmol/l. In conclusion, fat tissue vitamin D(3) can be measured by LC/MS and is detectable in obese subjects with suboptimal vitamin D status. Compatible with the long-standing concept that fat tissue is a storage site for vitamin D, fat tissue and serum vitamin D(3) concentrations were positively correlated.
描述脂肪组织中维生素D含量的文献极为有限。我们开展了一项初步研究，测量肥胖成年人脂肪组织和血清中维生素D(3)的浓度。这些测量采用了一种新的液相色谱质谱联用（LC/MS）方法。本研究的目的是：测量并报告肥胖个体血清和皮下脂肪样本中维生素D(3)的浓度，以及检测脂肪中的维生素D(3)与血清中维生素D(3)之间的关联。这项横断面研究纳入了17名计划接受胃旁路手术的肥胖男性和女性。受试者皮下脂肪组织样本中维生素D(3)的平均浓度为102.8±42.0 nmol/kg。血清中维生素D(3)的平均浓度为7.78±3.99 nmol/l。脂肪组织和血清中的维生素D(3)浓度呈正相关（r = 0.68，P = 0.003）。与先前对肥胖受试者的研究结果一致，本研究中的受试者维生素D状态欠佳，其平均25-羟维生素D浓度为43.3±15.4 nmol/l。总之，脂肪组织中的维生素D(3)可用LC/MS进行测量，且在维生素D状态欠佳的肥胖受试者中可检测到。与脂肪组织是维生素D储存部位这一长期概念相符，脂肪组织和血清中维生素D(3)的浓度呈正相关。
