Wilson N, Yakoleff V, Keeler R
Department of Physiology, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Am J Physiol. 1991 Oct;261(4 Pt 1):E516-24. doi: 10.1152/ajpendo.1991.261.4.E516.
A large molecular form of immunoreactive atrial natriuretic factor (irANF) was demonstrated in plasma of rabbit and rat on the basis of gel filtration experiments. This big ANF was not retained by octadecylsilane cartridges and cross-reacted with four anti-ANF antisera of different specificities. Gel filtration in acid, but not in 8 M urea, resulted in material with elution characteristics of irANF. Affinity chromatography and gel electrophoresis of big ANF suggested that the material was similar to albumin. However, high concentrations of big ANF were found in analbuminemic rats, with characteristics similar to those seen in rabbit and normal rats (affinity and gel chromatography and gel electrophoresis). We thus conclude that big ANF represents a bound form of ANF in circulation and that the carrier is similar to but not identical with albumin.
基于凝胶过滤实验，在兔和大鼠的血浆中证实了一种大分子形式的免疫反应性心房利钠因子（irANF）。这种大分子ANF不被十八烷基硅烷柱保留，并与四种不同特异性的抗ANF抗血清发生交叉反应。在酸性条件下而非8M尿素中进行凝胶过滤，得到具有irANF洗脱特性的物质。大分子ANF的亲和层析和凝胶电泳表明该物质与白蛋白相似。然而，在无白蛋白血症大鼠中发现了高浓度的大分子ANF，其特征与在兔和正常大鼠中观察到的相似（亲和、凝胶层析和凝胶电泳）。因此，我们得出结论，大分子ANF代表循环中ANF的一种结合形式，且载体与白蛋白相似但不相同。
