Wilson N, Yakoleff V, Claybaugh J R
Department of Physiology, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Am J Physiol. 1991 Oct;261(4 Pt 1):E525-8. doi: 10.1152/ajpendo.1991.261.4.E525.
A large-molecular-weight form of atrial natriuretic factor (ANF) was found in rabbit plasma. When extraction of plasma utilizing octadecylsilane cartridges is employed, this big ANF is not retained by the cartridge. When this material is dissociated with acid, immunoreactive ANF (irANF) is released. Of the acids tested, formic and acetic acid (0.05 M) were indicated as the most effective in causing the dissociation. High-performance liquid chromatography analysis of dissociated ANF, confirmed by coelution with synthetic peptides, disclosed the presence of ANF-(99-126) and ANF-(103-126) in a ratio of approximately 2:1. Atriopeptin I was detected in most of the samples analyzed, but atriopeptin II was only detected in one analysis. Despite the wide spectrum of the antiserum used for detection of irANF in the eluent fractions, only immunoreactive peaks coeluting with the four standards were detected. We therefore conclude that big ANF is a bound form of atrial peptides found in normal circulation.
在兔血浆中发现了一种大分子形式的心房利钠因子（ANF）。当采用十八烷基硅烷柱提取血浆时，这种大分子ANF不会被柱保留。当该物质用酸解离时，会释放出免疫反应性ANF（irANF）。在所测试的酸中，甲酸和乙酸（0.05M）被表明是引起解离最有效的。通过与合成肽共洗脱确认的解离ANF的高效液相色谱分析显示，ANF-(99 - 126)和ANF-(103 - 126)的存在比例约为2:1。在大多数分析的样本中检测到了心房肽I，但仅在一次分析中检测到了心房肽II。尽管用于检测洗脱液组分中irANF的抗血清具有广泛的谱，但仅检测到了与四种标准品共洗脱的免疫反应性峰。因此，我们得出结论，大分子ANF是正常循环中发现的心房肽的一种结合形式。
