红火蚁工蚁和幼虫中入侵红火蚁病毒2基因组及中间复制型病毒RNA的检测与定量分析
Detection and quantitation of Solenopsis invicta virus-2 genomic and intermediary replicating viral RNA in fire ant workers and larvae.
作者信息
Hashimoto Yoshifumi, Valles Steven M
机构信息
Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Drive, Gainesville, FL 32608, USA.
出版信息
J Invertebr Pathol. 2008 Jun;98(2):243-5. doi: 10.1016/j.jip.2008.02.002. Epub 2008 Feb 13.
Quantitative real-time PCR (QPCR) was used to quantify the genome of Solenopsis invicta virus-2 (SINV-2) from infected individual ants of S. invicta. Strand-specific cDNA synthesis oligonucleotide primers and RNase digestion after cDNA synthesis allowed quantification of plus (genomic) and minus (replicative) strands of the SINV-2 genome. Both strands were detected in adult workers and larval fire ants indicating that the virus was replicating within the ant. The differences between the genomic to replicative strand ranged from 199-fold in larvae to 479-fold in workers with an average ratio of 339:1.
采用定量实时PCR（QPCR）技术对感染红火蚁的红火蚁病毒2（SINV-2）基因组进行定量分析。链特异性cDNA合成寡核苷酸引物以及cDNA合成后的核糖核酸酶消化，实现了对SINV-2基因组正链（基因组链）和负链（复制链）的定量。在成年工蚁和幼虫红火蚁中均检测到了两条链，这表明该病毒在蚂蚁体内进行复制。基因组链与复制链的差异倍数在幼虫中为199倍，在工蚁中为479倍，平均比例为339:1。
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