Imported Fire Ant and Household Insects Research Unit, U.S. Department of Agriculture-Agricultural Research Service, 1600 SW 23rd Drive, Gainesville, FL 32608, USA.
J Invertebr Pathol. 2011 Jul;107(3):212-5. doi: 10.1016/j.jip.2011.04.005. Epub 2011 Apr 23.
A series of oligonucleotide primer pairs covering the entire genome of Solenopsis invicta virus 1 (SINV-1) were used to probe the genome of its host, S. invicta, for integrated fragments of the viral genome. All of the oligonucleotide primer sets yielded amplicons of anticipated size from cDNA created from an RNA template from SINV-1. However, no corresponding amplification was observed when genomic DNA (from 32 colonies of S. invicta) was used as template for the PCR amplifications. Host DNA integrity was verified by amplification of an ant-specific gene, SiGSTS1. The representation of fire ant colonies included both social forms, monogyne and polygyne, and those infected and uninfected with SINV-1. Furthermore, no amplification was observed from genomic DNA from ant samples collected from Argentina or the US. Thus, it appears that SINV-1 genome integration, or a portion therein, has not likely occurred within the S. invicta host genome.
使用一系列覆盖红火蚁病毒 1（SINV-1）全基因组的寡核苷酸引物对，探测其宿主红火蚁的基因组中是否整合了病毒基因组的片段。所有的寡核苷酸引物对都从 SINV-1 的 RNA 模板创建的 cDNA 中产生了预期大小的扩增子。然而，当使用基因组 DNA（来自 32 个红火蚁的菌落）作为 PCR 扩增的模板时，没有观察到相应的扩增。通过扩增抗特异性基因 SiGSTS1 来验证宿主 DNA 的完整性。所检测的红火蚁菌落代表了两种社会形式，单蚁后和多蚁后，以及感染和未感染 SINV-1 的红火蚁。此外，从来自阿根廷或美国的蚂蚁样本的基因组 DNA 中也没有观察到扩增。因此，似乎 SINV-1 基因组的整合，或其中的一部分，不太可能发生在红火蚁的宿主基因组中。
