Aoki Kazuhiro, Kiyokawa Etsuko, Nakamura Takeshi, Matsuda Michiyuki
Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
Philos Trans R Soc Lond B Biol Sci. 2008 Jun 27;363(1500):2143-51. doi: 10.1098/rstb.2008.2267.
Fluorescence probes based on the principle of Förster resonance energy transfer (FRET) have shed new light on our understanding of signal transduction cascades. Among them, unimolecular FRET probes containing fluorescence proteins are rapidly increasing in number because these genetically encoded probes can be easily loaded into living cells and allow simple acquisition of FRET images. We have developed probes for small GTPases, tyrosine kinases, serine-threonine kinases and phosphoinositides. Images obtained with these probes have revealed that membrane protrusions such as nascent lamellipodia or neurites provide an active signalling platform in the growth factor-stimulated cells.
基于福斯特共振能量转移（FRET）原理的荧光探针为我们理解信号转导级联反应带来了新的曙光。其中，含有荧光蛋白的单分子FRET探针数量正在迅速增加，因为这些基因编码的探针可以很容易地导入活细胞，并能简单地获取FRET图像。我们已经开发出了针对小GTP酶、酪氨酸激酶、丝氨酸 - 苏氨酸激酶和磷酸肌醇的探针。用这些探针获得的图像显示，诸如新生板状伪足或神经突等膜突出物在生长因子刺激的细胞中提供了一个活跃的信号平台。
