Tuena de Gómez-Puyou M, de Jesús García J, Gómez-Puyou A
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, D.F.
Biochemistry. 1993 Mar 9;32(9):2213-8. doi: 10.1021/bi00060a012.
Soluble F1 from beef heart that has been depleted of adenine nucleotides to values of 0.4 mol of ADP and 0.1 mol ATP/mol of enzyme has the capacity to synthetize about 0.1 mol of ATP/mol of enzyme from medium phosphate in the presence of Mg2+ and 30% dimethyl sulfoxide. Under the same conditions, native and adenine nucleotide depleted F1 can also synthesize pyrophosphate to values that range from 0.03 to 0.05 mol/mol of F1. The formation of pyrophosphate requires Mg2+ and dimethyl sulfoxide. The formed pyrophosphate remains bound to F1 during filtration through Sephadex centrifugation columns. In all water media, adenine nucleotide depleted, but not native, F1 can hydrolyze pyrophosphate to values of about 0.2 nmol min-1 mg-1. This activity is inhibited or stimulated by agents (adenylyl imidodiphosphate, aurovertin, and methanol) that produce such effects on the ATPase activity of F1; NaN3 stimulated the activity. Therefore, F1 from bovine heart mitochondria has the capacity to catalyze synthesis and hydrolysis of ATP. Synthesis of pyrophosphate by the soluble F1 appears to follow the same energetic considerations that have been postulated for ATP synthesis by the soluble enzyme [de Meis (1989) Biochim. Biophys. Acta 973, 339-349].
来自牛心的可溶性F1，其腺嘌呤核苷酸已被消耗至每摩尔酶含0.4摩尔ADP和0.1摩尔ATP，在存在Mg2+和30%二甲基亚砜的情况下，有能力从培养基中的磷酸盐合成每摩尔酶约0.1摩尔ATP。在相同条件下，天然的和腺嘌呤核苷酸耗尽的F1也能将焦磷酸合成至每摩尔F1为0.03至0.05摩尔的水平。焦磷酸的形成需要Mg2+和二甲基亚砜。在通过葡聚糖凝胶离心柱过滤过程中，形成的焦磷酸仍与F1结合。在所有水性介质中，腺嘌呤核苷酸耗尽而非天然的F1能将焦磷酸水解至约0.2 nmol min-1 mg-1的水平。这种活性受到对F1的ATP酶活性产生此类影响的试剂（腺苷酰亚胺二磷酸、金雀花碱和甲醇）的抑制或刺激；NaN3刺激该活性。因此，牛心线粒体的F1有能力催化ATP的合成和水解。可溶性F1合成焦磷酸似乎遵循与可溶性酶合成ATP所假定的相同的能量考虑因素[德梅斯（1989年）《生物化学与生物物理学报》973，339 - 349]。
