囊性纤维化患者痰液中存在铁载体。
Siderophore presence in sputa of cystic fibrosis patients.
作者信息
Haas B, Kraut J, Marks J, Zanker S C, Castignetti D
机构信息
Department of Biology, Loyola University of Chicago, Illinois 60626.
出版信息
Infect Immun. 1991 Nov;59(11):3997-4000. doi: 10.1128/iai.59.11.3997-4000.1991.
Abstract
Sputum samples from the lungs of cystic fibrosis patients harboring Pseudomonas aeruginosa infections were collected and examined for the presence of the siderophore pyoverdine. Fluorescence quenching, due to the addition of ferric ion, as well as column and thin-layer chromatography results indicated that all samples contained the siderophore. Six samples furnished sufficient material after purification to allow us to obtain visible absorbance spectra. These spectra were characteristic of the ferrated analog of the P. aeruginosa pyoverdine, that is, ferripyoverdine, and in all cases they indicated a degree of ferration in excess of 50%. P. aeruginosa in the cystic fibrosis lung is thus iron stressed and responds by synthesizing pyoverdine, which subsequently binds ferric ion.
摘要
收集了患有铜绿假单胞菌感染的囊性纤维化患者肺部的痰液样本，并检测其中是否存在铁载体绿脓菌素。由于添加了铁离子导致的荧光猝灭，以及柱色谱和薄层色谱结果表明，所有样本均含有该铁载体。六个样本在纯化后提供了足够的材料，使我们能够获得可见吸收光谱。这些光谱是铜绿假单胞菌绿脓菌素的铁结合类似物（即铁绿脓菌素）的特征光谱，并且在所有情况下都表明铁结合程度超过50%。因此，囊性纤维化肺部的铜绿假单胞菌处于铁应激状态，并通过合成绿脓菌素做出反应，随后绿脓菌素会结合铁离子。
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