Wilkinson J R, Lane S J, Lee T H
Department of Allergy and Allied Respiratory Disorders, Guy's Hospital, London, UK.
Int Arch Allergy Appl Immunol. 1991;94(1-4):220-1. doi: 10.1159/000235365.
The capacity of corticosteroids to inhibit the secretion of cytokines and the expression of selective antigens on monocytes has been studied in corticosteroid-sensitive (CS) and corticosteroid-resistant (CR) asthmatic patients. Incubation of monocytes derived from CS subjects with hydrocortisone inhibited the production of the enhancing activity, whereas in CR subjects hydrocortisone at concentrations of up to 10(-4) M did not suppress the release of enhancing activity. There was a rank order of potency for corticosteroid action: hydrocortisone less than methylprednisolone less than dexamethasone. The major activity was characterized as a heat-sensitive peptide of 3,000 daltons. The expression of CR1, CR3 and class II on asthmatic peripheral-blood mononuclear cells was increased relative to normal control donors. Culturing monocytes for 24 h in the presence of 10(-4) M hydrocortisone inhibited the expression of CR1, CR3 and class II in CS subjects but not in CR individuals. These results suggest that monocytes of CR asthmatic patients can increase the inflammatory potential of neutrophils and that they are hyperactive, as indicated by increased cytokine production and enhanced expression of activation markers, despite the presence of corticosteroids.
在皮质类固醇敏感（CS）和皮质类固醇抵抗（CR）的哮喘患者中，研究了皮质类固醇抑制细胞因子分泌和单核细胞上选择性抗原表达的能力。用氢化可的松孵育CS受试者来源的单核细胞可抑制增强活性的产生，而在CR受试者中，浓度高达10⁻⁴ M的氢化可的松并未抑制增强活性的释放。皮质类固醇作用的效力存在等级顺序：氢化可的松＜甲泼尼龙＜地塞米松。主要活性被表征为一种3000道尔顿的热敏感肽。与正常对照供体相比，哮喘外周血单个核细胞上CR1、CR3和II类分子的表达增加。在10⁻⁴ M氢化可的松存在下培养单核细胞24小时，可抑制CS受试者中CR1、CR3和II类分子的表达，但对CR个体无此作用。这些结果表明，CR哮喘患者的单核细胞可增加中性粒细胞的炎症潜能，并且尽管存在皮质类固醇，但其仍处于高活性状态，表现为细胞因子产生增加和活化标志物表达增强。
