Jacob Fabrício, Santos-Fortuna Elizabeth, Azevedo Raymundo Soares, Caterino-de-Araujo Adele
Immunology Department, Instituto Adolfo Lutz, Av. Dr. Arnaldo 355, 11 andar, 01246-902 São Paulo, SP, Brazil.
J Clin Virol. 2008 Jun;42(2):149-55. doi: 10.1016/j.jcv.2008.01.017. Epub 2008 Mar 17.
HTLV-1/2 diagnosis in high-risk populations from São Paulo, Brazil has been problematic due a high proportion of seroindeterminate results.
To confirm and extend previous findings regarding HTLV-1/2 diagnosis in this geographic area.
Sera from 2312 patients were tested for HTLV-1/2 antibodies using enzyme immunoassay (EIA) and Western blot (WB) analysis. Patients were from AIDS Reference Centers (Group I; 1393 patients) and HTLV out-patient clinics (Group II; 919 patients). Results were analyzed according to patients' age, gender, and clinic type.
HTLV-1 and HTLV-2 were detected in both groups. Among seropositive females, HTLV-2 was slightly more common in Group I (54.5%), while HTLV-1 prevailed in Group II (73.9%). Males from Group II had a higher percentage of HTLV-seroindeterminate results. No correlation between HTLV serological results and age was detected. Temporal analyses disclosed a high number of HTLV-seroindeterminate samples, and a large spectrum of indeterminate WB profiles. GD21 and/or rgp46-II bands were detected in 34.6% of sera from Group I, and a p24 or p19 band was detected in 35.3% of sera from Group II.
High rates of HTLV-indeterminate serological patterns during temporal analyses were confirmed in high-risk populations from São Paulo, Brazil.
由于血清学结果不确定的比例较高，巴西圣保罗高危人群的HTLV-1/2诊断一直存在问题。
证实并扩展此前关于该地理区域HTLV-1/2诊断的研究结果。
使用酶免疫测定（EIA）和蛋白质印迹（WB）分析对2312例患者的血清进行HTLV-1/2抗体检测。患者来自艾滋病参考中心（第一组；1393例患者）和HTLV门诊（第二组；919例患者）。根据患者的年龄、性别和诊所类型对结果进行分析。
两组均检测到HTLV-1和HTLV-2。在血清阳性女性中，HTLV-2在第一组中略为常见（54.5%），而HTLV-1在第二组中占主导（73.9%）。第二组男性的HTLV血清学结果不确定的比例更高。未检测到HTLV血清学结果与年龄之间的相关性。时间分析显示HTLV血清学结果不确定的样本数量较多，且WB图谱的不确定范围较广。在第一组34.6%的血清中检测到GD21和/或rgp46-II条带，在第二组35.3%的血清中检测到p24或p19条带。
在巴西圣保罗的高危人群中，时间分析期间HTLV血清学模式不确定的发生率较高得到了证实。
