通过共聚焦显微镜对球体进行成像及表面轮廓分析。

Imaging of spheres and surface profiling by confocal microscopy.

作者信息

Aguilar J F, Lera M, Sheppard C J

机构信息

Department of Physical Optics, School of Physics A 28, University of Sydney, Sydney, N.S.W. 2006, Australia.

出版信息

Appl Opt. 2000 Sep 1;39(25):4621-8. doi: 10.1364/ao.39.004621.

DOI:10.1364/ao.39.004621

PMID:18350052

Abstract

An investigation of the imaging of spheres can aid in understanding details of the surface profiling method of confocal microscopy. The use of semicircular masks to eliminate artifacts in confocal profiling is investigated experimentally. A theoretical treatment of image formation for a spherical object in reflection confocal microscopy is presented. For large spheres a simple approximate theory is described and is shown to be equivalent to application of the Kirchhoff theory for surface scattering.

摘要

对球体成像的研究有助于理解共聚焦显微镜表面轮廓测量方法的细节。通过实验研究了使用半圆形掩膜消除共聚焦轮廓测量中伪像的情况。给出了反射共聚焦显微镜中球形物体成像的理论处理方法。对于大球体，描述了一种简单的近似理论，并证明其等同于应用基尔霍夫表面散射理论。