Ryberg Kristina, Gruvberger Birgitta, Zimerson Erik, Isaksson Marléne, Persson Lena, Sörensen Osten, Goossens An, Bruze Magnus
Department of Occupational and Environmental Dermatology, Lund University, Malmö University Hospital, Malmö, Sweden.
Contact Dermatitis. 2008 Apr;58(4):199-209. doi: 10.1111/j.1600-0536.2007.01298.x.
Contact allergy to textile dyes is not uncommon. The allergy is detected by patch testing patients with commercial patch test preparations.
To investigate 8 disperse dyes (DDs) used for patch testing in the departments in Malmö and in Leuven and to compare them with test preparations used at various dermatology departments.
MATERIALS/METHODS: The investigated DDs were Disperse Blue (DB) 35, 106, and 124, Disperse Yellow (DY) 3, Disperse Orange (DO) 1 and 3, and Disperse Red (DR) 1 and 17. From 13 clinics, 107 petrolatum preparations were analysed using high-performance liquid chromatography and thin-layer chromatography (TLC), and compared with reference substances obtained at the Malmö laboratory. Concerning DB 35, no reference substance could be identified.
TLC visualized impurities in all DDs. For each DD, except DB 35, the mean concentration in the preparations labelled to contain 1.0% (w/w) were DB 106: 0.30%, DB 124: 0.25%, DY 3: 0.44%, DO 1: 0.40%, DO 3: 0.68%, DR 1: 0.49%, and DR 17: 0.35%; there were variations between the samples also with regard to the number of impurities. DO 3 could not be demonstrated in 4/15 preparations labelled DO 3.
The results may have implications for individual diagnosis and prevention and when comparing test results from various centres.
对纺织染料的接触性过敏并不罕见。通过使用商用斑贴试验制剂对患者进行斑贴试验来检测这种过敏反应。
调查在马尔默和鲁汶的各科室用于斑贴试验的8种分散染料（DDs），并将它们与各皮肤科使用的试验制剂进行比较。
材料/方法：所研究的分散染料有分散蓝（DB）35、106和124，分散黄（DY）3，分散橙（DO）1和3，以及分散红（DR）1和17。从13个诊所收集了107份凡士林制剂，采用高效液相色谱法和薄层色谱法（TLC）进行分析，并与在马尔默实验室获得的参考物质进行比较。关于DB 35，未能鉴定出参考物质。
薄层色谱法显示所有分散染料中均有杂质。对于每种分散染料，除DB 35外，标记含1.0%（w/w）的制剂中的平均浓度分别为：DB 106：0.30%，DB 124：0.25%，DY 3：0.44%，DO 1：0.40%，DO 3：0.68%，DR 1：0.49%，DR 17：0.35%；样品之间在杂质数量方面也存在差异。在15份标记为DO 3的制剂中，有4份未检测到DO 3。
这些结果可能对个体诊断和预防以及比较不同中心的检测结果具有重要意义。
