局部应用异黄酮可对皮肤提供有效的光保护作用。

Topical isoflavones provide effective photoprotection to skin.

作者信息

Lin Jing-Yi, Tournas Joshua A, Burch James A, Monteiro-Riviere Nancy A, Zielinski Jan

机构信息

Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taipei, Taiwan.

出版信息

Photodermatol Photoimmunol Photomed. 2008 Apr;24(2):61-6. doi: 10.1111/j.1600-0781.2008.00329.x.

DOI:10.1111/j.1600-0781.2008.00329.x

PMID:18353084

Abstract

BACKGROUND/PURPOSE: Isoflavones, one main group of phytoestrogens, have antioxidative and photoprotective effects in cellular and mouse studies. The aim of this study is to obtain a more comprehensive understanding of the isoflavone-mediated photoprotection with the pig skin model, a more human-resembling model.

METHODS

The pig skin was treated with five well-known isoflavone compounds (genistein, equol, daidzein, biochanin A, and formononetin) and one antioxidant combination solution of 15% vitamin C and 1% vitamin E and 0.5% ferulic acid (CEF) daily for 4 days. Skin was irradiated with solar-simulated UV irradiation, 1 to 5 minimal erythema dose (MED) at 1-MED intervals. Evaluation was carried out 24 h later by colorimeter-measured erythema and sunburn cell numbers.

RESULTS

Topical application of 0.5% solutions of three individual phytoestrogens - genistein, daidzein, biochanin A - are better than similar solutions of equol or formononetin in protecting pig skin from solar-simulated ultraviolet (SSUV)-induced photodamage, as measured by sunburn cell formation and/or erythema. However, the protection was less than that provided by a topical combination antioxidant standard containing 15% L-ascorbic acid, 1%alpha-tocopherol, and 0.5% ferulic acid.

CONCLUSION

Isoflavones provide effective photoprotection and are good candidate ingredients for protection against ultraviolet (UV) photodamage.

摘要

背景/目的：异黄酮是植物雌激素的主要类别之一，在细胞和小鼠研究中具有抗氧化和光保护作用。本研究的目的是通过猪皮肤模型（一种更接近人类的模型）更全面地了解异黄酮介导的光保护作用。

方法

用五种著名的异黄酮化合物（染料木黄酮、雌马酚、大豆苷元、鹰嘴豆芽素A和芒柄花黄素）以及一种含15%维生素C、1%维生素E和0.5%阿魏酸的抗氧化剂组合溶液（CEF）每日处理猪皮肤，持续4天。用模拟太阳紫外线以1最小红斑量（MED）的间隔照射皮肤1至5个MED。24小时后通过色度计测量红斑和晒伤细胞数量进行评估。

结果

通过晒伤细胞形成和/或红斑测量，三种单独的植物雌激素（染料木黄酮、大豆苷元、鹰嘴豆芽素A）的0.5%溶液局部应用在保护猪皮肤免受模拟太阳紫外线（SSUV）诱导的光损伤方面优于雌马酚或芒柄花黄素的类似溶液。然而，这种保护作用小于含15% L-抗坏血酸、1%α-生育酚和0.5%阿魏酸的局部抗氧化剂标准组合所提供的保护作用。