Darr D, Dunston S, Faust H, Pinnell S
North Carolina Biotechnology Center, Raleigh, N.C., USA.
Acta Derm Venereol. 1996 Jul;76(4):264-8. doi: 10.2340/0001555576264268.
Considerable interest has been recently generated concerning the use of natural compounds, anti-oxidants in particular, in photoprotection. Two of the best known anti-oxidants are vitamins C and E, both of which have been shown to be somewhat effective in different models of photodamage. Very little has been reported, however, on the effectiveness of a combination of the two (known to be biologically the more relevant situation); nor have there been detailed studies on the ability of these antioxidants to augment commercial sunscreen protection against UV damage. We report that (in swine skin) vitamin C is capable of additive protection against acute UVB damage (sunburn cell formation) when combined with a UVB sunscreen. A combination of both vitamins E and C provided very good protection from a UVB insult, the bulk of the protection attributable to vitamin E. However, vitamin C is significantly better than vitamin E at protecting against a UVA-mediated phototoxic insult in this animal model, while the combination is only slightly more effective than vitamin C alone. When vitamin C or a combination of vitamin C and E is formulated with a commercial UVA sunscreen (oxybenzone), an apparently greater than additive protection is noted against the phototoxic damage. These results confirm the utility of anti-oxidants as photoprotectants but suggest the importance of combining the compounds with known sunscreens to maximize photoprotection.
近期，人们对天然化合物尤其是抗氧化剂在光防护中的应用产生了浓厚兴趣。两种最知名的抗氧化剂是维生素C和维生素E，在不同的光损伤模型中，它们均已显示出一定的效果。然而，关于二者联用（已知在生物学上更具相关性）的效果报道极少；而且对于这些抗氧化剂增强商业防晒霜抵御紫外线损伤能力的研究也不够详尽。我们报告称（在猪皮中），维生素C与中波紫外线（UVB）防晒霜联用时，能够对急性UVB损伤（晒伤细胞形成）起到相加性防护作用。维生素E和维生素C联用对UVB侵害具有很好的防护效果，大部分防护作用归因于维生素E。然而，在该动物模型中，维生素C在抵御长波紫外线（UVA）介导的光毒性侵害方面明显优于维生素E，而二者联用仅比单独使用维生素C稍有效。当维生素C或维生素C与维生素E的组合与商业UVA防晒霜（二苯甲酰甲烷）配制成型时，可以观察到对光毒性损伤的防护作用明显大于相加性效果。这些结果证实了抗氧化剂作为光保护剂的效用，但也表明将这些化合物与已知的防晒霜联用对于实现最大化光保护的重要性。
