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2
Isolation and quantitation of long-chain acyl-coenzyme A esters in brain tissue by solid-phase extraction.通过固相萃取法分离和定量脑组织中的长链酰基辅酶A酯。
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A fast and versatile method for extraction and quantitation of long-chain acyl-CoA esters from tissue: content of individual long-chain acyl-CoA esters in various tissues from fed rat.一种从组织中提取和定量长链酰基辅酶A酯的快速通用方法：喂食大鼠不同组织中各长链酰基辅酶A酯的含量
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Simultaneous quantification of malonyl-CoA and several other short-chain acyl-CoAs in animal tissues by ion-pairing reversed-phase HPLC/MS.采用离子对反相高效液相色谱/质谱联用技术同时定量分析动物组织中丙二酰辅酶A及其他几种短链酰基辅酶A。
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Determination of short-chain acyl-coenzyme A esters by high-performance liquid chromatography.通过高效液相色谱法测定短链酰基辅酶A酯
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Malonyl-CoA and long chain acyl-CoA esters as metabolic coupling factors in nutrient-induced insulin secretion.丙二酰辅酶A和长链酰基辅酶A酯作为营养诱导胰岛素分泌中的代谢偶联因子。
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本文引用的文献

1
Quantification of malonyl-coenzyme A in tissue specimens by high-performance liquid chromatography/mass spectrometry.采用高效液相色谱/质谱法对组织标本中的丙二酰辅酶A进行定量分析。
Anal Biochem. 2006 May 1;352(1):24-32. doi: 10.1016/j.ab.2006.02.015. Epub 2006 Mar 2.
2
Quantification of the concentration and 13C tracer enrichment of long-chain fatty acyl-coenzyme A in muscle by liquid chromatography/mass spectrometry.通过液相色谱/质谱法对肌肉中长链脂肪酰辅酶A的浓度和13C示踪剂丰度进行定量分析。
Anal Biochem. 2006 Feb 1;349(1):87-95. doi: 10.1016/j.ab.2005.10.006. Epub 2005 Oct 26.
3
Orthogonality of separation in two-dimensional liquid chromatography.二维液相色谱中分离的正交性。
Anal Chem. 2005 Oct 1;77(19):6426-34. doi: 10.1021/ac050923i.
4
LC/MS/MS method for quantitative determination of long-chain fatty acyl-CoAs.用于定量测定长链脂肪酰辅酶A的液相色谱-串联质谱法
Anal Chem. 2005 May 1;77(9):2889-94. doi: 10.1021/ac048314i.
5
Strategy for the isolation, derivatization, chromatographic separation, and detection of carnitine and acylcarnitines.肉碱和酰基肉碱的分离、衍生化、色谱分离及检测策略。
Anal Chem. 2005 Mar 1;77(5):1448-57. doi: 10.1021/ac0487810.
6
Determination of long-chain fatty acid acyl-coenzyme A compounds using liquid chromatography-electrospray ionization tandem mass spectrometry.使用液相色谱-电喷雾电离串联质谱法测定长链脂肪酸酰基辅酶A化合物
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Sep 5;808(2):263-8. doi: 10.1016/j.jchromb.2004.05.012.
7
Analysis of medium-chain acyl-coenzyme A esters in mouse tissues by liquid chromatography-electrospray ionization mass spectrometry.通过液相色谱-电喷雾电离质谱法分析小鼠组织中的中链酰基辅酶A酯。
Anal Biochem. 2004 Feb 15;325(2):196-205. doi: 10.1016/j.ab.2003.10.043.
8
Separation and quantitation of short-chain coenzyme A's in biological samples by capillary electrophoresis.通过毛细管电泳对生物样品中的短链辅酶A进行分离和定量分析。
Anal Chem. 2003 Jan 1;75(1):78-82. doi: 10.1021/ac0261505.

从组织中分离短链、中链和长链酰基辅酶A酯的新型方法。

Novel isolation procedure for short-, medium-, and long-chain acyl-coenzyme A esters from tissue.

作者信息

Minkler Paul E, Kerner Janos, Ingalls Stephen T, Hoppel Charles L

机构信息

Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.

出版信息

Anal Biochem. 2008 May 15;376(2):275-6. doi: 10.1016/j.ab.2008.02.022. Epub 2008 Feb 29.

DOI:10.1016/j.ab.2008.02.022
PMID:18355435
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2444051/
Abstract

A novel procedure for the quantitative isolation and purification of acyl-coenzyme A esters is presented. The procedure involves two steps: (1) tissue extraction using acetonitrile/2-propanol (3+1, v+v) followed by 0.1M potassium phosphate, pH 6.7, and (2) purification using 2-(2-pyridyl)ethyl-functionalized silica gel. Recoveries determined by adding radiolabeled acetyl-, malonyl-, octanoyl-, oleoyl-, palmitoyl-, or arachidonyl-coenzyme A to powdered rat liver varied 93-104% for tissue extraction and 83-90% for solid-phase extraction. The procedure described allows for isolation and purification, with high recoveries, of acyl-coenzyme A esters differing widely in chain length and saturation.

摘要

本文介绍了一种用于定量分离和纯化酰基辅酶A酯的新方法。该方法包括两个步骤：（1）先用乙腈/2-丙醇（3+1，v/v），然后用pH 6.7的0.1M磷酸钾进行组织提取；（2）用2-（2-吡啶基）乙基功能化硅胶进行纯化。通过向大鼠肝脏粉末中添加放射性标记的乙酰辅酶A、丙二酰辅酶A、辛酰辅酶A、油酰辅酶A、棕榈酰辅酶A或花生四烯酰辅酶A来测定回收率，组织提取的回收率在93%-104%之间，固相提取的回收率在83%-90%之间。所述方法能够以高回收率分离和纯化链长和饱和度差异很大的酰基辅酶A酯。