Wang Han, Kesinger Jason W, Zhou Qingchun, Wren Jonathan D, Martin George, Turner Sean, Tang Yuhong, Frank Mark Barton, Centola Michael
Department of Zoology and Stephenson Research and Technology Center, University of Oklahoma, 101 David L. Boren Boulevard, Norman, OK 73019, USA.
Genome. 2008 Mar;51(3):222-35. doi: 10.1139/G07-098.
To study genes that are specifically expressed in the eyes, we employed microarray and in situ hybridization analyses to identify and characterize differentially expressed ocular genes in eyeless masterblind (mbl-/-) zebrafish (Danio rerio). Among 70 differentially expressed genes in the mbl-/- mutant identified by microarray analysis, 8 down-regulated genes were characterized, including 4 eye-specific genes, opsin 1 short-wave-sensitive 1 (opn1sw1), crystallinbetaa1b (cryba1b), crystallinbetaa2b (cryba2b), and crystallingamma M2d3 (crygm2d3); 2 eye and brain genes, ATPase, H+ transporting, lysosomal, V0 subunit c (atp6v0c) and basic leucine zipper and W2 domains 1a (bzw1a); and 2 constitutive genes, heat shock protein 8 (hspa8) and ribosomal protein L7a (rpl7a). In situ hybridization experiments confirmed down-regulation of these 8 ocular formation genes in mbl-/- zebrafish and showed their ocular and dynamic temporal expression patterns during zebrafish early development. Further, an automated literature analysis of the 70 differentially expressed genes identified a sub-network of genes with known associations, either with each other or with ocular structures or development, and shows how this study contributes to the current body of knowledge.
为了研究在眼睛中特异性表达的基因，我们采用微阵列和原位杂交分析来鉴定和表征无眼主盲（mbl-/-）斑马鱼（Danio rerio）中差异表达的眼部基因。在通过微阵列分析鉴定出的mbl-/-突变体中的70个差异表达基因中，对8个下调基因进行了表征，包括4个眼特异性基因，视蛋白1短波敏感1（opn1sw1）、晶状体βa1b（cryba1b）、晶状体βa2b（cryba2b）和晶状体γM2d3（crygm2d3）；2个眼和脑基因，ATP酶，H+转运，溶酶体，V0亚基c（atp6v0c）和碱性亮氨酸拉链和W2结构域1a（bzw1a）；以及2个组成型基因，热休克蛋白8（hspa8）和核糖体蛋白L7a（rpl7a）。原位杂交实验证实了这8个眼部形成基因在mbl-/-斑马鱼中的下调，并显示了它们在斑马鱼早期发育过程中的眼部和动态时间表达模式。此外，对这70个差异表达基因的自动文献分析确定了一个具有已知关联的基因子网络，这些关联要么是相互之间的，要么是与眼部结构或发育的关联，并展示了这项研究如何为当前的知识体系做出贡献。
