Kim M S, McMurtrey J E, Mulchi C L, Daughtry C S, Chappelle E W, Chen Y R
Appl Opt. 2001 Jan 1;40(1):157-66. doi: 10.1364/ao.40.000157.
We present a detailed description of a laboratory-based multispectral fluorescence imaging system (MFIS) for plant leaves. Fluorescence emissions with 360-nm excitation are captured at four spectral bands in the blue, green, red, and far-red regions of the spectrum centered at 450, 550, 680, and 740 nm, respectively. Preliminary experiments conducted with soybean leaves treated with a herbicide (DCMU) and short-term exposures to moderately elevated tropospheric ozone environment demonstrated the utilities of the newly developed MFIS. In addition, with the aid of fluorescence images of normal soybean leaves, several mechanisms governing the fluorescence emissions are discussed. Imaging results illustrate the versatility of fluorescence imaging, which provides information on the spatial variability of fluorescence patterns over leaf samples.
我们详细介绍了一种用于植物叶片的基于实验室的多光谱荧光成像系统（MFIS）。用360纳米激发光激发产生的荧光发射分别在光谱的蓝色、绿色、红色和远红色区域的四个光谱带中捕获，中心波长分别为450、550、680和740纳米。对用除草剂（二氯苯基二甲基脲）处理过的大豆叶片以及在对流层臭氧环境适度升高的情况下进行短期暴露的大豆叶片进行的初步实验证明了新开发的MFIS的实用性。此外，借助正常大豆叶片的荧光图像，讨论了几种控制荧光发射的机制。成像结果说明了荧光成像的多功能性，它提供了关于叶片样本上荧光模式空间变异性的信息。
