Zhengzhou Institute of Multipurpose Utilization of Mineral Resources, CAGS, Zhengzhou, 450006, China.
China National Engineering Research Center for Utilization of Industrial Minerals, Zhengzhou, 450006, China.
Anal Sci. 2021 Feb 10;37(2):261-266. doi: 10.2116/analsci.20P075. Epub 2020 May 15.
N-Benzylquininium chloride is a versatile functional monomer with quinoline and benzyl groups, which is beneficial for reversed-phase chromatography. In this study, a novel monolithic column with reversed-phase mode was synthesized using N-benzylquininium chloride as the monomer and 3-(acryloyloxy)-2-hydroxypropyl methacrylate as the cross-linker in a binary porogenic solvent consisting of PEG 400 and a 0.05 M sodium hydroxide aqueous solution. The alkaline solution were found to be useful for the improvement of the mechanical stability of the porous monoliths. The monolithic column showed excellent reversed-phase selectivity and various compounds, such as alkylbenzenes, phenols and polycyclic aromatic hydrocarbons, were separated successfully. The highest column efficiency was 1.75 × 10 N m. The relative standard deviations of the migration time for thiourea and four alkylbenzenes were all less than 5.0%, which indicates the monolithic column has good stability. The application of the monolithic column for the analysis of polycyclic aromatic hydrocarbons in spiked lake water samples illustrated its great potential for practical application.
N-苄基奎宁盐酸盐是一种具有喹啉和苄基基团的多功能功能单体，有利于反相色谱。在这项研究中，使用 N-苄基奎宁盐酸盐作为单体，3-(丙烯酰氧基)-2-羟丙基甲基丙烯酸酯作为交联剂，在由 PEG 400 和 0.05 M 氢氧化钠水溶液组成的二元致孔溶剂中合成了一种新型的反相模式整体柱。碱性溶液有利于提高多孔整体柱的机械稳定性。该整体柱表现出优异的反相选择性，能够成功分离烷基苯、酚类和多环芳烃等多种化合物。最高柱效为 1.75×10 N m。硫脲和四种烷基苯的迁移时间的相对标准偏差均小于 5.0%，表明该整体柱具有良好的稳定性。该整体柱在加标湖水样品中多环芳烃分析中的应用表明了其在实际应用中的巨大潜力。
