Ecotoxicology Section, Industrial Toxicology Research Centre, Post Box No. 80, M.G. Marg, Lucknow 226001, India.
Ecotoxicol Environ Saf. 2008 Nov;71(3):711-6. doi: 10.1016/j.ecoenv.2008.02.003. Epub 2008 Mar 20.
Cytogenetic and DNA damaging effects of cadmium (Cd) were examined through chromosome aberrations (CA) and Comet assay on the root meristem cells of Allium cepa. Root meristem cells were exposed to Cd (10, 20, and 40 μM) for 24h and also left in water for 24h recovery. Exposure of Cd revealed significant (P<0.001) inhibition of mitotic index (MI), induction of CA, mitotic aberrations (MA), and micronucleus (MN) formation. Similarly, at 40 μM significant increase in the tail length (85.70 ± 9.40 μm) and tail moment (58.75 ± 5.30 μm) was observed as compared to control 4.50 ± 0.76 and 0.86 ± 0.03 μm, respectively. However, cells examined at 24h post-exposure showed concentration-dependent decline in all the endpoints. Findings of this study confirms the root meristem cells of A. cepa are suitable model for detecting both the environmentally induced CA as well as DNA damage analyzed by Comet assay.
通过对洋葱根尖细胞的染色体畸变（CA）和彗星试验，研究了镉（Cd）的细胞遗传学和 DNA 损伤效应。将根尖细胞暴露于 Cd（10、20 和 40 μM）24 小时，并在水中恢复 24 小时。暴露于 Cd 显著抑制有丝分裂指数（MI）（P<0.001），诱导 CA、有丝分裂异常（MA）和微核（MN）形成。同样，与对照（4.50 ± 0.76 和 0.86 ± 0.03 μm）相比，在 40 μM 时观察到尾长（85.70 ± 9.40 μm）和尾矩（58.75 ± 5.30 μm）显著增加（P<0.001）。然而，暴露后 24 小时检测到的细胞所有终点都呈浓度依赖性下降。本研究的结果证实，洋葱根尖细胞是一种合适的模型，可用于检测环境诱导的 CA 以及彗星试验分析的 DNA 损伤。
