Abd El Mageed Shimaa A, Sayed Ali A S, Shaaban Ahmed, Hemida Khaulood A, Abdelkhalik Abdelsattar, Semida Wael M, Mohamed Ibrahim A A, Gyushi Mohammed A H, Elmohsen Yasmine H Abd, Abd El Mageed Taia A
Agronomy Department, Faculty of Agriculture, Fayoum University, Fayoum, 63514, Egypt.
Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, 63514, Egypt.
BMC Plant Biol. 2025 Jan 8;25(1):26. doi: 10.1186/s12870-024-05954-0.
Globally, salinity poses a threat to crop productivity by hindering plant growth and development via osmotic stress and ionic cytotoxicity. Plant extracts have lately been employed as exogenous adjuvants to improve endogenous plant defense mechanisms when grown under various environmental stresses, such as salinity. This study investigated the potential of melatonin (Mt; 0, 50, and 100 mM) as an antioxidant and licorice root extract (LRE; 0.0 and 3%) as an organic biostimulant applied sequentially as a foliar spray on faba bean (Vicia faba L.) grown in cadmium (Cd)-contaminated saline soil conditions [Cd = 4.71 (mg kg soil) and ECe = 7.84 (dS m)]. Plants not receive any treatment and sprayed with HO were considered controls. The experimental treatments were laid out in strip plot in a randomized complete block design replicated thrice, where the LRE and Mt were considered as vertical and horizontal strips, respectively. Growth characteristics, photosynthetic pigments, nutrient uptake, physiology and metabolic responses, anatomical features, and yield were assessed.
Cadmium (Cd) and salinity-induced stress significantly altered leaf integrity, photosynthetic efficiency, total soluble sugars (TSS), free proline (FPro), total phenolic, DPPH, and total soluble proteins (TSP), non-enzymatic and enzymatic antioxidants, growth characteristics and yield-related traits. However, the application of LRE + Mt considerably improved these negative effects, with higher improvements were observed due to application of LRE + Mt. Application of LRE + Mt significantly reduced hydrogen peroxide (HO) accumulation, lipid peroxidation and Cd content in leaves and seeds, all of which had increased due to Cd stress. Application of LRE + Mt significantly mitigated the Cd-induced oxidative damage by increasing the activity of reactive oxygen species (ROS) scavenging enzymes such as superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase, in parallel with enhanced ascorbate and reducing glutathione content. Exogenous application of LRE + Mt significantly increased osmolyte content, including FPro, TSS, and total phenols and mitigated Cd-induced reduction to considerable levels.
Our findings showed that LRE + Mt increased V. faba plants' morphological, physiological, and biochemical properties, reducing Cd stress toxicity, and promoting sustainable agricultural practices.
Not applicable.
在全球范围内，盐度通过渗透胁迫和离子细胞毒性阻碍植物生长发育，对作物生产力构成威胁。植物提取物最近被用作外源佐剂，以改善植物在各种环境胁迫（如盐度）下生长时的内源性防御机制。本研究调查了褪黑素（Mt；0、50和100 mM）作为抗氧化剂以及甘草根提取物（LRE；0.0和3%）作为有机生物刺激剂的潜力，将它们依次作为叶面喷雾剂施用于生长在镉（Cd）污染盐渍土壤条件[Cd = 4.71（mg/kg土壤）和ECe = 7.84（dS/m）]下的蚕豆（Vicia faba L.）。未接受任何处理并喷洒水的植物被视为对照。实验处理采用随机完全区组设计的条区试验，重复三次，其中LRE和Mt分别视为垂直条区和水平条区。评估了生长特性、光合色素、养分吸收、生理和代谢反应、解剖特征及产量。
镉（Cd）和盐度诱导的胁迫显著改变了叶片完整性、光合效率、总可溶性糖（TSS）、游离脯氨酸（FPro）、总酚、DPPH和总可溶性蛋白（TSP）、非酶和酶抗氧化剂、生长特性及产量相关性状。然而，LRE + Mt的施用显著改善了这些负面影响，且LRE + Mt的施用带来了更高的改善效果。LRE + Mt的施用显著降低了过氧化氢（H₂O₂）积累、脂质过氧化以及叶片和种子中的Cd含量，这些因Cd胁迫均有所增加。LRE + Mt的施用通过提高超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶和谷胱甘肽还原酶等活性氧（ROS）清除酶的活性，同时提高抗坏血酸和还原型谷胱甘肽含量，显著减轻了Cd诱导的氧化损伤。LRE + Mt的外源施用显著增加了渗透调节物质含量，包括FPro、TSS和总酚，并将Cd诱导的降低减轻到相当程度。
我们的研究结果表明，LRE + Mt提高了蚕豆植株的形态、生理和生化特性，降低了Cd胁迫毒性，并促进了可持续农业实践。
不适用。
