Zhang Shiqing, Li Dexue, Li Enzhong, Li Lan, Wang Junjun, Wang Changyong, Lu Jingyan, Zhang Xueming
Institute of Basic Medical Sciences, Beijing 100850, People's Republic of China.
Mol Cell Endocrinol. 2008 Jun 11;287(1-2):47-56. doi: 10.1016/j.mce.2008.01.008. Epub 2008 Jan 29.
B cell-specific Moloney murine leukaemia virus integration site 1 (Bmi1), known as the first functional member of PcG (Polycomb Group) family, is supposed to be a key regulator of stem cell self-proliferation. In this study, we investigated its expression in testis and its impact on spermatogonia proliferation for better understanding of its role in spermatogenesis. Results showed that Bmi1 was expressed in undifferentiated spermatogonia (A(s), A(pr) and A(al) spermatogonia). Overexpression of BMI1 could promote spermatogonia proliferation, while repression of endogenous Bmi1 by RNAi resulted in inhibition of the proliferation.
B细胞特异性莫洛尼鼠白血病病毒整合位点1（Bmi1），作为聚梳蛋白家族（PcG）的首个功能性成员，被认为是干细胞自我增殖的关键调节因子。在本研究中，我们调查了其在睾丸中的表达及其对精原细胞增殖的影响，以更好地了解其在精子发生中的作用。结果显示，Bmi1在未分化的精原细胞（A(s)、A(pr)和A(al)精原细胞）中表达。BMI1的过表达可促进精原细胞增殖，而RNA干扰对内源性Bmi1的抑制则导致增殖受到抑制。
