Rasheed Zafar
Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh-202002, U.P., India.
Clin Biochem. 2008 Jun;41(9):663-9. doi: 10.1016/j.clinbiochem.2008.02.013. Epub 2008 Mar 18.
The role of hydroxyl radical (OH) damaged Immunoglobulin G (IgG) in rheumatoid arthritis (RA) has been investigated.
The study was hypothesized that oxidative by-products, like OH-damage IgG, help to initiate autoimmunity in RA. To test this hypothesis, IgG was modified by OH. Immunogenicity of native and modified IgG was probed by inducing polyclonal antibodies in rabbits. Autoantibodies from 77 RA sera were screened by direct binding and competition ELISA.
The OH caused extensive damage to IgG. The OH-IgG was found to be highly immunogenic in rabbits as compare to native IgG. High degree of specific binding by 72.7% RA sera autoantibodies towards OH-IgG was observed, in comparison to its native analogue (p<0.05).
The OH modification of IgG causes perturbations, resulting in the generation of neo-epitopes, and making it a potential immunogen. The IgG modified with the .OH may be one of the factors for the induction of circulating RA autoantibodies.
研究羟自由基（OH）损伤的免疫球蛋白G（IgG）在类风湿关节炎（RA）中的作用。
本研究假设氧化副产物，如OH损伤的IgG，有助于引发RA中的自身免疫。为验证该假设，用OH修饰IgG。通过在兔体内诱导多克隆抗体来检测天然和修饰后IgG的免疫原性。采用直接结合和竞争ELISA法筛选77例RA血清中的自身抗体。
OH对IgG造成广泛损伤。与天然IgG相比，OH-IgG在兔体内具有高度免疫原性。与天然类似物相比，观察到72.7%的RA血清自身抗体对OH-IgG有高度特异性结合（p<0.05）。
IgG的OH修饰引起扰动，导致新表位的产生，使其成为潜在的免疫原。经.OH修饰的IgG可能是诱导循环RA自身抗体的因素之一。
