胶体的三维共聚焦显微镜检查

Three-dimensional confocal microscopy of colloids.

作者信息

Dinsmore A D, Weeks E R, Prasad V, Levitt A C, Weitz D A

出版信息

Appl Opt. 2001 Aug 20;40(24):4152-9. doi: 10.1364/ao.40.004152.

DOI:10.1364/ao.40.004152

Abstract

Confocal microscopy is used in the study of colloidal gels, glasses, and binary fluids. We measure the three-dimensional positions of colloidal particles with a precision of approximately 50 nm (a small fraction of each particle's radius) and with a time resolution sufficient for tracking the thermal motions of several thousand particles at once. This information allows us to characterize the structure and the dynamics of these materials in qualitatively new ways, for example, by quantifying the topology of chains and clusters of particles as well as by measuring the spatial correlations between particles with high mobilities. We describe our experimental technique and describe measurements that complement the results of light scattering.

摘要

共聚焦显微镜用于研究胶体凝胶、玻璃和二元流体。我们测量胶体颗粒的三维位置，精度约为50纳米（每个颗粒半径的一小部分），时间分辨率足以同时跟踪数千个颗粒的热运动。这些信息使我们能够以全新的定性方式表征这些材料的结构和动力学，例如，通过量化颗粒链和簇的拓扑结构以及测量高迁移率颗粒之间的空间相关性。我们描述了我们的实验技术，并描述了补充光散射结果的测量。