Normann Andrea, Badur Selim, Onel Derya, Kilic Ayse, Sidal Müjgan, Larouzé Bernard, Massari Veronique, Müller Julia, Flehmig Bertram
University of Tübingen, Children's Hospital, Tübingen, Germany.
J Med Virol. 2008 May;80(5):785-90. doi: 10.1002/jmv.21137.
Anti-HAV IgM positive serum samples from acute phase hepatitis A patients from various areas in Turkey were tested for viral RNA by RT-PCR (reverse transcriptase polymerase chain reaction), using primer pairs from two different regions of the HAV genome. The PCR products amplified from both genomic regions underwent phylogenetic analyses. A comparison of the regions showed the same genotyping results, and the RT-PCR-2 in the 5'NCR demonstrated greater sensitivity compared to RT-PCR-1 in the VP1-P2A region. The majority of the isolates belonged to genotype IB and are related closely to each other; however, two isolates related even more strongly to the HAV HM175 strain. Two (n = 37) RT-PCR positive sera were classified under genotype IA. A surprising finding emerged for the mean levels of serum transaminases AST and ALT: higher levels were found in patients under 10 years of age compared to older patients. Anti-HAV IgM levels were determined quantitatively and, in addition, the HAV-RNA genome equivalents were ascertained by real time RT-PCR. No evidence was found for an association between viral load and the higher transaminase levels in the younger group.
利用来自甲型肝炎病毒（HAV）基因组两个不同区域的引物对，通过逆转录聚合酶链反应（RT-PCR）对来自土耳其不同地区的急性甲型肝炎患者急性期的抗-HAV IgM阳性血清样本进行病毒RNA检测。从两个基因组区域扩增得到的PCR产物进行了系统发育分析。对这些区域的比较显示出相同的基因分型结果，并且5'非编码区（5'NCR）的RT-PCR-2与VP1-P2A区域的RT-PCR-1相比表现出更高的灵敏度。大多数分离株属于基因型IB，并且彼此密切相关；然而，有两个分离株与HAV HM175毒株的关系更为密切。两份（共37份）RT-PCR阳性血清被归类为基因型IA。血清转氨酶AST和ALT的平均水平出现了一个惊人的发现：10岁以下患者的水平高于老年患者。对抗-HAV IgM水平进行了定量测定，此外，通过实时RT-PCR确定了HAV-RNA基因组当量。未发现年轻组中病毒载量与较高转氨酶水平之间存在关联的证据。
