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Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics.转录因子 IIS 和 IIF 通过不同方式修饰 RNA 聚合酶暂停动力学，从而增强转录效率。
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本文引用的文献

1
The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes.转录延伸因子TFIIS是RNA聚合酶II起始前复合物的一个组成部分。
Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16068-73. doi: 10.1073/pnas.0704573104. Epub 2007 Oct 3.
2
The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex.TFIIF和TFIIE在RNA聚合酶II转录起始前复合物中的位置。
Nat Struct Mol Biol. 2007 Aug;14(8):696-703. doi: 10.1038/nsmb1272. Epub 2007 Jul 15.
3
A DNA-tethered cleavage probe reveals the path for promoter DNA in the yeast preinitiation complex.一种DNA连接的切割探针揭示了酵母预起始复合物中启动子DNA的路径。
Nat Struct Mol Biol. 2006 Jul;13(7):603-10. doi: 10.1038/nsmb1117. Epub 2006 Jul 2.
4
Genetic interactions between TFIIF and TFIIS.TFIIF与TFIIS之间的遗传相互作用。
Genetics. 2006 Aug;173(4):1871-84. doi: 10.1534/genetics.106.058834. Epub 2006 Apr 30.
5
Quantitative analysis of in vivo initiator selection by yeast RNA polymerase II supports a scanning model.酵母RNA聚合酶II体内起始子选择的定量分析支持扫描模型。
J Biol Chem. 2006 May 19;281(20):14119-28. doi: 10.1074/jbc.M601937200. Epub 2006 Mar 29.
6
Evidence that the Tfg1/Tfg2 dimer interface of TFIIF lies near the active center of the RNA polymerase II initiation complex.TFIIF的Tfg1/Tfg2二聚体界面位于RNA聚合酶II起始复合物活性中心附近的证据。
Nucleic Acids Res. 2005 Sep 7;33(16):5045-52. doi: 10.1093/nar/gki825. Print 2005.
7
A functional role for the switch 2 region of yeast RNA polymerase II in transcription start site utilization and abortive initiation.酵母RNA聚合酶II的开关2区域在转录起始位点利用和流产起始中的功能作用。
J Biol Chem. 2005 Oct 14;280(41):34917-23. doi: 10.1074/jbc.M502932200. Epub 2005 Aug 4.
8
Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE.利用5' SAGE技术对酿酒酵母转录起始位点进行定位
Nucleic Acids Res. 2005 May 19;33(9):2838-51. doi: 10.1093/nar/gki583. Print 2005.
9
Anc1 interacts with the catalytic subunits of the general transcription factors TFIID and TFIIF, the chromatin remodeling complexes RSC and INO80, and the histone acetyltransferase complex NuA3.Anc1与通用转录因子TFIID和TFIIF的催化亚基、染色质重塑复合物RSC和INO80以及组蛋白乙酰转移酶复合物NuA3相互作用。
Biochem Biophys Res Commun. 2005 Jul 1;332(2):398-403. doi: 10.1016/j.bbrc.2005.04.158.
10
Evidence that the elongation factor TFIIS plays a role in transcription initiation at GAL1 in Saccharomyces cerevisiae.有证据表明延伸因子TFIIS在酿酒酵母中GAL1基因的转录起始过程中发挥作用。
Mol Cell Biol. 2005 Apr;25(7):2650-9. doi: 10.1128/MCB.25.7.2650-2659.2005.

酿酒酵母TFIIF在转录起始位点利用过程中的功能。

Functions of Saccharomyces cerevisiae TFIIF during transcription start site utilization.

作者信息

Khaperskyy Denys A, Ammerman Michelle L, Majovski Robert C, Ponticelli Alfred S

机构信息

Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York, Buffalo, NY 14214-3000, USA.

出版信息

Mol Cell Biol. 2008 Jun;28(11):3757-66. doi: 10.1128/MCB.02272-07. Epub 2008 Mar 24.

DOI:10.1128/MCB.02272-07
PMID:18362165
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2423299/
Abstract

Previous studies have shown that substitutions in the Tfg1 or Tfg2 subunits of Saccharomyces cerevisiae transcription factor IIF (TFIIF) can cause upstream shifts in start site utilization, resulting in initiation patterns that more closely resemble those of higher eukaryotes. In this study, we report the results from multiple biochemical assays analyzing the activities of wild-type yeast TFIIF and the TFIIF Tfg1 mutant containing the E346A substitution (Tfg1-E346A). We demonstrate that TFIIF stimulates formation of the first two phosphodiester bonds and dramatically stabilizes a short RNA-DNA hybrid in the RNA polymerase II (RNAPII) active center and, importantly, that the Tfg1-E346A substitution coordinately enhances early bond formation and the processivity of early elongation in vitro. These results are discussed within a proposed model for the role of yeast TFIIF in modulating conformational changes in the RNAPII active center during initiation and early elongation.

摘要

先前的研究表明，酿酒酵母转录因子IIF（TFIIF）的Tfg1或Tfg2亚基中的取代可导致起始位点利用的上游偏移，从而产生更类似于高等真核生物的起始模式。在本研究中，我们报告了多项生化分析的结果，这些分析检测了野生型酵母TFIIF和含有E346A取代的TFIIF Tfg1突变体（Tfg1-E346A）的活性。我们证明，TFIIF刺激前两个磷酸二酯键的形成，并显著稳定RNA聚合酶II（RNAPII）活性中心的短RNA-DNA杂交体，重要的是，Tfg1-E346A取代在体外协同增强早期键的形成和早期延伸的持续性。我们在一个关于酵母TFIIF在起始和早期延伸过程中调节RNAPII活性中心构象变化作用的模型中讨论了这些结果。