无猝灭剂分子信标：基于荧光的核酸分析新策略。

Quencher-free molecular beacons: a new strategy in fluorescence based nucleic acid analysis.

作者信息

Venkatesan Natarajan, Seo Young Jun, Kim Byeang Hyean

机构信息

Laboratory for Modified Nucleic Acid Systems, Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, Korea.

出版信息

Chem Soc Rev. 2008 Apr;37(4):648-63. doi: 10.1039/b705468h. Epub 2008 Jan 17.

DOI:10.1039/b705468h

PMID:18362974

Abstract

Molecular beacons (MBs) have been used as viable fluorescent probes in nucleic acid analysis. Many researchers around the world continue to modify the MBs to suit their needs. As a result, a number of nucleic acid probing systems with close resemblance to the MBs are being reported from time to time. Quencher-free molecular beacons (QF-MBs) are a significant modification of the conventional MB; in QF-MBs the quencher part has been eliminated. Despite the absence of the quencher, the QF-MBs can identify specific target DNA. They can also be used in SNP typing and in real-time PCR analysis for quantification of DNAs. The design, factors behind functioning and applications of different types of QF-MBs and closely related quencher-free nucleic acid probing systems (QF-NAPs) have been described in this tutorial review.

摘要

分子信标（MBs）已被用作核酸分析中可行的荧光探针。世界各地的许多研究人员不断对分子信标进行修改以满足他们的需求。因此，不时有一些与分子信标极为相似的核酸探测系统被报道。无猝灭剂分子信标（QF-MBs）是对传统分子信标的重大改进；在无猝灭剂分子信标中，猝灭剂部分已被去除。尽管没有猝灭剂，但无猝灭剂分子信标仍可识别特定的目标DNA。它们还可用于单核苷酸多态性（SNP）分型以及DNA定量的实时聚合酶链反应（PCR）分析。本综述教程描述了不同类型的无猝灭剂分子信标以及与之密切相关的无猝灭剂核酸探测系统（QF-NAPs）的设计、作用原理及应用。