Department of Mechanical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Anal Chem. 2012 Jul 17;84(14):5939-43. doi: 10.1021/ac3004727. Epub 2012 Jul 2.
In this work, we propose a simple and enzyme-free strategy for sensitive and selective DNA detection by using two different types of molecular beacons (MBs), MB1 and MB2. In this method, the target DNA binds with and restores the fluorescence of MB1 first. Then, MB2 hybridizes with MB1 and free the target, which is used to trigger another reaction cycle. The cycling use of the target and the employment of bi-MBs amplify the fluorescence intensity for sensitive DNA detection. The detection limit of this method was obtained as 10 pM, which is about 2 orders of magnitude sensitive than the conventional MB-based approaches.
在这项工作中，我们提出了一种简单且无需酶的策略，通过使用两种不同类型的分子信标（MB），即 MB1 和 MB2，来实现灵敏和选择性的 DNA 检测。在该方法中，靶 DNA 首先与 MB1 结合并恢复其荧光。然后，MB2 与 MB1 杂交并释放出靶 DNA，从而触发另一个反应循环。靶 DNA 的循环利用和双 MB 的使用使荧光强度得以放大，从而实现了灵敏的 DNA 检测。该方法的检测限达到了 10 pM，比传统的基于 MB 的方法灵敏了约 2 个数量级。
