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相移数字全息术中的图像形成及其在显微镜中的应用。

Image formation in phase-shifting digital holography and applications to microscopy.

作者信息

Yamaguchi I, Kato J, Ohta S, Mizuno J

出版信息

Appl Opt. 2001 Dec 1;40(34):6177-86. doi: 10.1364/ao.40.006177.

DOI:10.1364/ao.40.006177
PMID:18364918
Abstract

We discuss image formation in phase-shifting digital holography by developing an analytical formulation based on the Fresnel-Kirchhoff diffraction theory. Image-plane position and imaging magnification are derived for general configurations in which a spherical reference is employed. The influences of discrete sampling of the resulting interference patterns by a CCD and numerical reconstruction on qualities of point images are investigated. Dependence of the point images on the ratio of the minimum fringe spacing to pixel pitch of the CCD is numerically analyzed. Two-point resolution and magnification are also investigated as a function of pixel numbers by a simulation using a one-dimensional model. In experiments magnified images of biological objects and a resolution target were reconstructed with the same quality as by conventional microscopy.

摘要

我们通过基于菲涅耳-基尔霍夫衍射理论开发一种解析公式来讨论相移数字全息术中的图像形成。对于采用球面参考的一般配置,推导了像平面位置和成像放大率。研究了电荷耦合器件(CCD)对所得干涉图样的离散采样以及数值重建对点图像质量的影响。通过数值分析了点图像对CCD最小条纹间距与像素间距之比的依赖性。还通过使用一维模型的模拟研究了两点分辨率和放大率作为像素数量的函数。在实验中,重建了生物物体和分辨率目标的放大图像,其质量与传统显微镜相同。

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