Cuche E, Marquet P, Depeursinge C
Institute of Applied Optics, Swiss Federal Institute of Technology, CH-1015 Lausanne, Switzerland.
Appl Opt. 1999 Dec 1;38(34):6994-7001. doi: 10.1364/ao.38.006994.
We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation. For phase-contrast imaging the reconstruction method involves the computation of a digital replica of the reference wave. A digital method for the correction of the phase aberrations is presented. We present a detailed description of the reconstruction procedure and show that the transverse resolution is equal to the diffraction limit of the imaging system.
我们提出了一种用于全息显微镜的数字方法，该方法使用电荷耦合器件（CCD）相机作为记录设备。用显微镜物体的放大图像记录的离轴全息图，通过在菲涅耳近似下计算标量衍射，在幅度和相位上进行数值重建。对于相衬成像，重建方法涉及参考波数字副本的计算。提出了一种用于校正相位像差的数字方法。我们对重建过程进行了详细描述，并表明横向分辨率等于成像系统的衍射极限。
