Abramova S L, Riazantsev D Iu, Voinova T M, Zavriev S K
Bioorg Khim. 2008 Jan-Feb;34(1):107-13. doi: 10.1134/s1068162008010135.
A PCR system in the fluorescent amplification-based specific hybridization (FLASH) format was developed for the detection and identification of two important wheat pathogenic fungi Septoria tritici (teleomorph of Mycosphaerella graminicola and Stagonospora nodorum (teleomorph of Phaeosphaeria nodorum), which cause spots on leaves and glumes, respectively. The pathogen detection system is based on the amplification of a genome fragment in the internal transcribed spacer 1 (ITS 1) region and a site encoding the 5.8S ribosomal RNA. The forward primers to ITS1 and a universal reverse primer and a Beacon type probe to the 5.8S ribosomal RNA region were chosen to provide the detection of the products in the FLASH format. This system was tested on different isolates of the pathogens, and on infected soil, leaf, and seed samples.
开发了一种基于荧光扩增的特异性杂交（FLASH）形式的PCR系统，用于检测和鉴定两种重要的小麦致病真菌，即小麦壳针孢（Mycosphaerella graminicola的有性型）和小麦叶枯病菌（Phaeosphaeria nodorum的有性型），它们分别在叶片和颖片上引起斑点。该病原体检测系统基于对内部转录间隔区1（ITS 1）区域的基因组片段和编码5.8S核糖体RNA的位点进行扩增。选择针对ITS1的正向引物、通用反向引物以及针对5.8S核糖体RNA区域的信标型探针，以实现对FLASH形式产物的检测。该系统在病原体的不同分离株以及受感染的土壤、叶片和种子样本上进行了测试。
