紧密连接蛋白-2在胃癌发生多阶段过程中的表达

Expression of claudin-2 in the multistage process of gastric carcinogenesis.

作者信息

Song X, Li X, Tang Y, Chen H, Wong B, Wang J, Chen M

机构信息

Department of Gastroenterology, the First Affiliated Hospital, Sun-Yat Sen University, Guangzhou, China.

出版信息

Histol Histopathol. 2008 Jun;23(6):673-82. doi: 10.14670/HH-23.673.

DOI:10.14670/HH-23.673

PMID:18366005

Abstract

OBJECTIVE

Claudin-2 is an important component of the tight junction (TJ) of epithelial cells, and its protein expression between many tumours is significantly different. It is unclear if Claudin-2 overexpression is a trigger or a consequence during carcinogenesis. The multistage tissues of gastric carcinogenesis provides us a valuable model for determining tumourigenicity.

METHODS

This paper investigated, for the first time, claudin-2 expression in the pathological paraffin tissues of sinus ventriculi from gastroscopic biopsy. To determine claudin-2 expression in gastric carcinogenesis by immunochemical ABC technique.

RESULTS

Altogether, 108 chronic superficial gastritis, 55 chronic atrophic gastritis, 109 intestinal-type metaplasia, 93 dysplasia and 52 gastric intestinal-type adenocarcinoma samples were analyzed. Results indicated that the percentage of claudin-2-positive cases was 0% for chronic superficial gastritis (0/108), 0% for chronic atrophic gastritis (0/55), 0% for intestinal-type metaplasia (0/109), 35.87% for dysplasia (33/92), and 73.47% for gastric intestinal-type adenocarcinoma (36/49) respectively, primarily in the cell membrane, and gradually increased in the multistage process of gastric carcinogenesis (P<0.001).

CONCLUSIONS

This suggests that claudin-2 protein overexpression may be closely correlated to gastric carcinogenesis.

摘要

目的

Claudin-2是上皮细胞紧密连接（TJ）的重要组成部分，其在多种肿瘤之间的蛋白表达存在显著差异。目前尚不清楚Claudin-2过表达在致癌过程中是触发因素还是结果。胃癌发生的多阶段组织为我们提供了一个确定肿瘤发生能力的宝贵模型。

方法

本文首次研究了胃镜活检胃窦病理石蜡组织中Claudin-2的表达情况。采用免疫化学ABC技术确定Claudin-2在胃癌发生中的表达。

结果

共分析了108例慢性浅表性胃炎、55例慢性萎缩性胃炎、109例肠化生、93例发育异常和52例胃肠型腺癌样本。结果显示，慢性浅表性胃炎Claudin-2阳性病例百分比为0%（0/108），慢性萎缩性胃炎为0%（0/55），肠化生为0%（0/109），发育异常为35.87%（33/92），胃肠型腺癌为73.47%（36/49），主要位于细胞膜，且在胃癌发生的多阶段过程中逐渐增加（P<0.001）。