Hu Y Q, Brown J W, Waugh R, Turner P C
Department of Biochemistry, University of Liverpool, U.K.
Biochim Biophys Acta. 1991 Dec 2;1129(1):90-2. doi: 10.1016/0167-4781(91)90217-a.
Using a mixed U6 snRNA gene probe and a low stringency hybridization procedure we have isolated a U6 snRNA containing clone from a potato genomic library in lambda EMBL 3. This clone contains a single U6 snRNA gene which has been subcloned and sequenced. Southern blotting experiments using this gene and the heterologous U6 genes as probes indicate that the potato U6 gene family consists of more than 20 members. The potato U6 gene sequence shows high identity to previously characterised plant U6 snRNA gene sequences and possesses correctly positioned and spaced transcription control elements suggesting that it is an active gene.
我们使用混合的U6小核仁RNA（snRNA）基因探针和低严谨度杂交程序，从λEMBL 3载体的马铃薯基因组文库中分离出一个含有U6 snRNA的克隆。该克隆包含一个单一的U6 snRNA基因，已将其亚克隆并测序。使用该基因和异源U6基因作为探针的Southern杂交实验表明，马铃薯U6基因家族由20多个成员组成。马铃薯U6基因序列与先前鉴定的植物U6 snRNA基因序列具有高度同一性，并拥有定位和间距正确的转录控制元件，表明它是一个活性基因。
