Wieben E D, Vrabel A M, Holicky E L, Klisak I, Sparkes R S, Stanford D R
Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, MN 55905.
Nucleic Acids Res. 1991 Jun 11;19(11):2869-74. doi: 10.1093/nar/19.11.2869.
A complex locus on human chromosome 1 brings together sequences homologous to a G protein and two components of the RNA processing machinery of eukaryotic cells. Specifically, the seventh intron of the human Gi3 alpha gene contains a fusion of a partial snRNP E protein pseudogene to a variant U6 snRNA gene. The novel U6 sequence contains nine point mutations and a one nucleotide deletion relative to the major U6 genes from humans. Unlike all other vertebrate U6 genes characterized to date, the variant U6 gene is efficiently transcribed by RNA polymerase III even in the absence of all natural flanking sequences. The union of elements from the signal transduction pathway and the RNA processing machinery suggests the possibility of functional interplay.
人类1号染色体上的一个复杂基因座汇集了与一种G蛋白以及真核细胞RNA加工机制的两个组分同源的序列。具体而言，人类Gi3α基因的第七个内含子包含一个部分snRNP E蛋白假基因与一个变异U6 snRNA基因的融合。相对于人类主要的U6基因，这个新的U6序列含有九个点突变和一个单核苷酸缺失。与迄今所鉴定的所有其他脊椎动物U6基因不同，即使在没有所有天然侧翼序列的情况下，这个变异U6基因也能被RNA聚合酶III高效转录。信号转导途径元件与RNA加工机制的结合暗示了功能相互作用的可能性。
