Pesheva M, Krastanova O, Stamenova R, Kantardjiev D, Venkov P
Faculty of Biology, Sofia University, 8 "Dragan Tsankov" Blvd, 1421 Sofia, Bulgaria.
Arch Toxicol. 2008 Oct;82(10):779-85. doi: 10.1007/s00204-008-0299-5. Epub 2008 Mar 28.
The Ty1 assay is a short-term test for detection of genotoxins based on induction of the transposition of a gene-engineered Ty1 retrotransposon in Saccharomyces cerevisiae cells. Here, we provide evidence that the Ty1 test responds positively in concentration-dependent manner to the carcinogenic genotoxins benz(a)anthracene, benzo(a)pyrene, chenodeoxycholic and taurodeoxycholic free bile acids and to environmental soil samples polluted with carcinogenic substances. The Ty1 test gives negative results with the noncarcinogenic mutagens benz(b)anthracene, benzo(e)pyrene, lithocholic and taurodeoxycholic conjugated bile acids and to soil samples not polluted with carcinogens. Presence or absence of genotoxins in soil samples was evidenced by chemical analysis. Several explanations for the sensitive differential test's response to genotoxins are proposed and discussed. It is concluded that the Ty1 test can complement existing assays in laboratory and environmental studies showing high sensitivity to a wider spectrum of carcinogenic genotoxins.
Ty1检测是一种基于基因工程改造的Ty1逆转座子在酿酒酵母细胞中转座诱导情况来检测基因毒素的短期试验。在此，我们提供证据表明，Ty1试验对致癌基因毒素苯并(a)蒽、苯并(a)芘、鹅去氧胆酸和牛磺鹅去氧胆酸游离胆汁酸以及被致癌物质污染的环境土壤样本呈浓度依赖性阳性反应。Ty1试验对非致癌诱变剂苯并(b)蒽、苯并(e)芘、石胆酸和牛磺石胆酸结合胆汁酸以及未被致癌物污染的土壤样本呈阴性结果。通过化学分析证实了土壤样本中基因毒素的存在与否。针对该敏感差异试验对基因毒素的反应提出并讨论了几种解释。得出的结论是，Ty1试验可以在实验室和环境研究中补充现有检测方法，对更广泛的致癌基因毒素表现出高敏感性。
