Najiah M, Nadirah M, Lee K L, Lee S W, Wendy W, Ruhil H H, Nurul F A
Faculty of Agrotechnology and Food Sciences, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia.
Vet Res Commun. 2008 Jun;32(5):377-81. doi: 10.1007/s11259-008-9045-y. Epub 2008 Mar 28.
Slipper oyster Crassostrea iredalei is a species of good demand for its sweet flavor and white coloured flesh. The filter feeding nature predisposes oysters to accumulation of pathogenic and heavy metals in waters impacted by sewage pollutions and may thus render the oysters unfit for human consumption. A study was undertaken to investigate the presence of bacteria flora and heavy metal concentrations in cultivated oysters Crassostrea iredalei at Setiu Wetland, Terengganu, the only source of cultivated oysters in East Coast of Malaysia. A total of 200 slipper oyster samples were analyzed. The bacteria were isolated using non selective agar such as TSA agar and selective agars before they were then identified using conventional methods in combination with BBL Crystal identification kit. Heavy metals such as zinc (Zn), copper (Cu), cadmium (Cd), and lead (Pb) concentrations were determined using atomic absorption spectrophotometry. Results showed that the oysters harbor predominantly Shewanella putrifaciens followed by Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholerae, Enterobacter cloacae, Escherichia coli and Chromobacterium violaceum. They also contain high concentration of Zn (785.68 +/- 285.88 microg/g) with the lowest heavy metal was Pb (0.17 +/- 0.15 microg/g), whilst the concentrations of other heavy metals were Cu (38.9 +/- 13.2 microg/g) and Cd (1.60 +/- 0.28 microg/g). The study is very useful to evaluate the type of bacteria and heavy metal present in oyster meat for human consumption.
拖鞋牡蛎（Crassostrea iredalei）因其甜美的味道和白色的肉质而备受青睐。滤食性的特点使牡蛎容易在受污水污染影响的水域中积累病原体和重金属，从而可能使牡蛎不适于人类食用。本研究旨在调查马来西亚东海岸唯一的养殖牡蛎产地——登嘉楼州Setiu湿地养殖的Crassostrea iredalei牡蛎中细菌菌群的存在情况和重金属浓度。共分析了200个拖鞋牡蛎样本。使用非选择性琼脂（如TSA琼脂）和选择性琼脂分离细菌，然后结合BBL Crystal鉴定试剂盒采用常规方法对其进行鉴定。使用原子吸收分光光度法测定锌（Zn）、铜（Cu）、镉（Cd）和铅（Pb）等重金属的浓度。结果表明，牡蛎中主要含有腐败希瓦氏菌，其次是副溶血性弧菌、创伤弧菌、霍乱弧菌、阴沟肠杆菌、大肠埃希氏菌和紫色色杆菌。它们还含有高浓度的锌（785.68 +/- 285.88微克/克），重金属含量最低的是铅（0.17 +/- 0.15微克/克），而其他重金属的浓度分别为铜（38.9 +/- 13.2微克/克）和镉（1.60 +/- 0.28微克/克）。该研究对于评估供人类食用的牡蛎肉中存在的细菌类型和重金属非常有用。
