Tsukamoto Hide, Mkrtchyan Hasmik, Dynnyk Alla
Research Center for Alcoholic Liver and Pancreatic Diseases and Cirrhosis, Department of Pathology, Keck School of Medicine of the University of Southern California, Los Angeles, CA, USA.
Methods Mol Biol. 2008;447:33-48. doi: 10.1007/978-1-59745-242-7_3.
Alcohol-associated life-style disease, as exemplified by alcoholic liver disease (ALD), is multifactorial with intricate interactions among genetic and environmental factors predicating individual predisposition. To experimentally dissect the interfaces of these interactions for better understanding of the pathogenesis, it is essential to have an animal model that provides maximal control over ethanol and dietary intake and that enables a precise addition or deletion analysis for a risk or protective factor of interest. Rodent intragastric ethanol infusion (IEI) model was developed two decades ago to meet this requirement. Work conducted with the model to date demonstrates the importance of both maximal ethanol intake and secondary risk factors in ALD. Mouse IEI model proved to be particularly useful for genetic analysis of the ALD pathogenesis and has the potential of producing synergistic pathologic outcome by combination of risk factors. The model is best used by alcohol researchers through a center-supported core facility and its tissue sharing program.
以酒精性肝病（ALD）为例的酒精相关生活方式疾病是多因素的，遗传和环境因素之间存在复杂的相互作用，决定了个体的易感性。为了通过实验剖析这些相互作用的界面，以便更好地理解发病机制，拥有一个能够对乙醇和饮食摄入进行最大程度控制，并能够对感兴趣的风险或保护因素进行精确的添加或缺失分析的动物模型至关重要。啮齿动物胃内乙醇灌注（IEI）模型是二十年前为满足这一需求而开发的。迄今为止，使用该模型进行的研究表明，最大乙醇摄入量和次要风险因素在ALD中都很重要。小鼠IEI模型被证明对ALD发病机制的遗传分析特别有用，并且有可能通过风险因素的组合产生协同病理结果。酒精研究人员最好通过中心支持的核心设施及其组织共享计划来使用该模型。
